Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   SD440_RS12280 Genome accession   NZ_CP139561
Coordinates   2361427..2361720 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus altitudinis strain NCCP     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2356427..2366720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD440_RS12230 sinR 2356591..2356926 (+) 336 WP_017357978.1 helix-turn-helix domain-containing protein Regulator
  SD440_RS12235 - 2356994..2357794 (-) 801 WP_008342195.1 TasA family protein -
  SD440_RS12240 - 2357853..2358425 (-) 573 WP_017357979.1 signal peptidase I -
  SD440_RS12245 tapA 2358475..2359002 (-) 528 WP_073413951.1 amyloid fiber anchoring/assembly protein TapA -
  SD440_RS12250 - 2359255..2359581 (+) 327 WP_056705243.1 DUF3889 domain-containing protein -
  SD440_RS12255 - 2359616..2359810 (-) 195 WP_008342191.1 YqzE family protein -
  SD440_RS12260 comGG 2359871..2360254 (-) 384 WP_035702776.1 competence type IV pilus minor pilin ComGG -
  SD440_RS12265 comGF 2360251..2360748 (-) 498 WP_041091745.1 competence type IV pilus minor pilin ComGF -
  SD440_RS12270 comGE 2360690..2361004 (-) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  SD440_RS12275 comGD 2360988..2361434 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  SD440_RS12280 comGC 2361427..2361720 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  SD440_RS12285 comGB 2361737..2362777 (-) 1041 WP_017357986.1 competence type IV pilus assembly protein ComGB -
  SD440_RS12290 comGA 2362758..2363828 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  SD440_RS12295 - 2364294..2365163 (-) 870 WP_007501231.1 STAS domain-containing protein -
  SD440_RS12305 - 2365389..2365769 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  SD440_RS12310 - 2365982..2366224 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=910155 SD440_RS12280 WP_008342186.1 2361427..2361720(-) (comGC) [Bacillus altitudinis strain NCCP]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=910155 SD440_RS12280 WP_008342186.1 2361427..2361720(-) (comGC) [Bacillus altitudinis strain NCCP]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTTATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAAGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392