Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SD440_RS12290 Genome accession   NZ_CP139561
Coordinates   2362758..2363828 (-) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain NCCP     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2357758..2368828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD440_RS12240 - 2357853..2358425 (-) 573 WP_017357979.1 signal peptidase I -
  SD440_RS12245 tapA 2358475..2359002 (-) 528 WP_073413951.1 amyloid fiber anchoring/assembly protein TapA -
  SD440_RS12250 - 2359255..2359581 (+) 327 WP_056705243.1 DUF3889 domain-containing protein -
  SD440_RS12255 - 2359616..2359810 (-) 195 WP_008342191.1 YqzE family protein -
  SD440_RS12260 comGG 2359871..2360254 (-) 384 WP_035702776.1 competence type IV pilus minor pilin ComGG -
  SD440_RS12265 comGF 2360251..2360748 (-) 498 WP_041091745.1 competence type IV pilus minor pilin ComGF -
  SD440_RS12270 comGE 2360690..2361004 (-) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  SD440_RS12275 comGD 2360988..2361434 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  SD440_RS12280 comGC 2361427..2361720 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  SD440_RS12285 comGB 2361737..2362777 (-) 1041 WP_017357986.1 competence type IV pilus assembly protein ComGB -
  SD440_RS12290 comGA 2362758..2363828 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  SD440_RS12295 - 2364294..2365163 (-) 870 WP_007501231.1 STAS domain-containing protein -
  SD440_RS12305 - 2365389..2365769 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  SD440_RS12310 - 2365982..2366224 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  SD440_RS12315 - 2366273..2366911 (-) 639 WP_251185767.1 MBL fold metallo-hydrolase -
  SD440_RS12320 - 2367072..2367245 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  SD440_RS12325 - 2367270..2367584 (-) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=910156 SD440_RS12290 WP_017357987.1 2362758..2363828(-) (comGA) [Bacillus altitudinis strain NCCP]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=910156 SD440_RS12290 WP_017357987.1 2362758..2363828(-) (comGA) [Bacillus altitudinis strain NCCP]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTTTCTACATTACCGACTGTAAATGATGAAAGTTTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATCCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCGAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGTGAATAC
GCTCATTCGTCATACGAAACGCTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649