Detailed information NTG0909637

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SPM78_RS29465
• Genome accession: NZ_CP139424
• Coordinates: 6278398..6279546 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain 136790
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6273398..6284546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPM78_RS29440 (SPM78_29440)	-	6274549..6274845 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
SPM78_RS29445 (SPM78_29445)	-	6274842..6275435 (-)	594	WP_003084544.1	YggT family protein	-
SPM78_RS29450 (SPM78_29450)	proC	6275446..6276267 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
SPM78_RS29455 (SPM78_29455)	-	6276279..6276971 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
SPM78_RS29460 (SPM78_29460)	pilT	6277186..6278220 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
SPM78_RS29465 (SPM78_29465)	pilU	6278398..6279546 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
SPM78_RS29470 (SPM78_29470)	czcD	6279554..6280453 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
SPM78_RS29475 (SPM78_29475)	-	6280608..6281012 (+)	405	WP_003084560.1	NINE protein	-
SPM78_RS29480 (SPM78_29480)	-	6281255..6282628 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
SPM78_RS29485 (SPM78_29485)	-	6282625..6283809 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=909637 SPM78_RS29465 WP_003110016.1 6278398..6279546(+) (pilU) [Pseudomonas aeruginosa strain 136790]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=909637 SPM78_RS29465 WP_003110016.1 6278398..6279546(+) (pilU) [Pseudomonas aeruginosa strain 136790]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCATG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382