Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   L6431_RS20655 Genome accession   NZ_AP025273
Coordinates   4339817..4341007 (-) Length   396 a.a.
NCBI ID   WP_419468750.1    Uniprot ID   -
Organism   Aquipseudomonas alcaligenes strain MRCP2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4331499..4339792 4339817..4341007 flank 25


Gene organization within MGE regions


Location: 4331499..4341007
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6431_RS20600 - 4331499..4332110 (+) 612 WP_237045170.1 hypothetical protein -
  L6431_RS20605 - 4332199..4332642 (+) 444 WP_237045171.1 hypothetical protein -
  L6431_RS20610 - 4332722..4332964 (+) 243 WP_237045172.1 hypothetical protein -
  L6431_RS20615 - 4333063..4333677 (+) 615 WP_237045173.1 hypothetical protein -
  L6431_RS20620 - 4333878..4334378 (+) 501 WP_021702784.1 hypothetical protein -
  L6431_RS20625 - 4334505..4334963 (+) 459 WP_237045174.1 hypothetical protein -
  L6431_RS20630 - 4335078..4335476 (+) 399 WP_237045175.1 DUF2628 domain-containing protein -
  L6431_RS20635 - 4335752..4336795 (-) 1044 WP_237044284.1 IS110-like element ISKpn4 family transposase -
  L6431_RS20640 - 4337149..4338420 (-) 1272 WP_004423958.1 IS4-like element ISPa1635 family transposase -
  L6431_RS20645 - 4338582..4338908 (+) 327 WP_237045176.1 hypothetical protein -
  L6431_RS20650 - 4339199..4339792 (+) 594 WP_237045177.1 hypothetical protein -
  L6431_RS20655 pilU 4339817..4341007 (-) 1191 WP_419468750.1 PilT/PilU family type 4a pilus ATPase Machinery gene

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43771.04 Da        Isoelectric Point: 6.1308

>NTDB_id=90928 L6431_RS20655 WP_419468750.1 4339817..4341007(-) (pilU) [Aquipseudomonas alcaligenes strain MRCP2]
MLKILSSQDGSDLYLSTGAPPCAKFNGVLKPLSQEPLKPGDVAAIANSVMDAEQRAEFEKELEMNLAISVPNVGRFRINI
FKQRNEVSIVARNIKMDIPKFEDLKLPEVLLQTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIEDPVEYV
HRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRIINFFP
EERRPQLLNDLGNNLRAFVSQRLVKTVDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQALIDLVH
EGAIDEEEAVKNADSANNVRLKLKLYKDNPAAPQAAAPAPAPVAAPTAAPANWAMELKLEEIEEDTPPEDPGRPSF

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=90928 L6431_RS20655 WP_419468750.1 4339817..4341007(-) (pilU) [Aquipseudomonas alcaligenes strain MRCP2]
ATGCTGAAAATCCTGTCCAGCCAGGACGGATCCGACCTCTACCTGTCCACCGGGGCACCGCCCTGCGCCAAGTTCAATGG
CGTGCTCAAGCCGCTCAGCCAGGAGCCGCTCAAGCCGGGTGACGTCGCCGCCATCGCCAATAGCGTGATGGACGCCGAAC
AGCGCGCCGAGTTCGAGAAGGAGCTGGAGATGAACCTGGCCATCTCGGTGCCCAATGTCGGCCGGTTCCGCATCAATATC
TTCAAACAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGATGGACATCCCCAAGTTCGAGGACCTCAAGCTGCC
CGAGGTGCTGCTGCAGACGGTGATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGGACCGGCTCGGGCAAGTCGACCT
CCCTGGCGGCGCTGATCGACTACCGCAACCGCAATAGCGGCGGCCACATCATCACCATCGAGGACCCGGTGGAGTACGTG
CACCGGCACAAGAAGTCGATCATCAACCAGCGTGAAGTCGGCGTGGACACCCGCAGCTTCCACGCCGCGCTGAAGAACAC
CCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACGCCCTGGCCTTCGCCG
ACACCGGCCACCTGGCGATCTCCACCCTGCATGCCAACAATGCCAACCAGGCGTTGGACCGCATCATCAACTTCTTCCCC
GAAGAGCGCCGCCCGCAGCTGCTCAACGACCTCGGCAACAACCTGCGCGCCTTCGTCTCCCAGCGCCTGGTCAAGACCGT
CGATGGCAAGCGCCGCGCCGCCGTAGAGGTGATGCTCGGCACGCCGACCGTACGCGACCTGATCAAGCGCAACGAGTTCT
CCGAGCTCAAGGAGATCATGGAGAAGTCGAAGAACCTCGGCATGCAGACCTTCGACCAGGCGCTGATCGACCTCGTCCAT
GAAGGCGCCATCGACGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCCAACAACGTGCGCCTCAAGCTCAAGCTGTACAA
GGACAACCCCGCTGCCCCGCAGGCTGCCGCACCGGCGCCCGCACCCGTTGCCGCGCCCACGGCGGCACCGGCCAACTGGG
CGATGGAGCTCAAGCTCGAGGAAATCGAGGAAGACACCCCTCCGGAAGACCCCGGCCGCCCGAGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.647

87.374

0.495
  pilU Acinetobacter baylyi ADP1

51.585

87.626

0.452
  pilU Vibrio cholerae strain A1552

52.802

85.606

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.992

95.202

0.371

Multiple sequence alignment