Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABS748_RS03580 Genome accession   NZ_CP158426
Coordinates   485421..486659 (+) Length   412 a.a.
NCBI ID   WP_192459655.1    Uniprot ID   -
Organism   Escherichia coli strain NRZ14408     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 480421..491659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS748_RS03555 (ABS748_03555) mrcA 480701..483253 (-) 2553 WP_001301905.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  ABS748_RS03560 (ABS748_03560) hofM 483373..484152 (+) 780 WP_001306315.1 DNA utilization protein HofM -
  ABS748_RS03565 (ABS748_03565) hofN 484152..484691 (+) 540 WP_021513263.1 DNA utilization protein HofN -
  ABS748_RS03570 (ABS748_03570) hofO 484675..485115 (+) 441 WP_001590819.1 DNA utilization protein HofO -
  ABS748_RS03575 (ABS748_03575) hofP 485105..485509 (+) 405 WP_001560037.1 DNA utilization protein HofP -
  ABS748_RS03580 (ABS748_03580) pilQ 485421..486659 (+) 1239 WP_192459655.1 DNA uptake porin HofQ Machinery gene
  ABS748_RS03585 (ABS748_03585) aroK 487060..487581 (+) 522 WP_000818618.1 shikimate kinase AroK -
  ABS748_RS03590 (ABS748_03590) aroB 487638..488726 (+) 1089 WP_000439850.1 3-dehydroquinate synthase -
  ABS748_RS03595 (ABS748_03595) damX 488818..490104 (+) 1287 WP_001590817.1 cell division protein DamX -
  ABS748_RS03600 (ABS748_03600) dam 490211..491047 (+) 837 WP_000742139.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44738.24 Da        Isoelectric Point: 5.9488

>NTDB_id=909183 ABS748_RS03580 WP_192459655.1 485421..486659(+) (pilQ) [Escherichia coli strain NRZ14408]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSIAWQNDNIARQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLTDAQQAGGVGQVTTLGSDLSVATATTHVGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=909183 ABS748_RS03580 WP_192459655.1 485421..486659(+) (pilQ) [Escherichia coli strain NRZ14408]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTGATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTGTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATCTAACAGATGTTCCCTGGAAGCAAGCACTACAAACTGTAGTGAAAAGCGCCGGACTG
ATAACGCGTCAGGAGGGCAACATTCTCTCAGTGCATTCCATTGCCTGGCAGAATGACAATATCGCCCGTCAGGAGGCGGA
GCAGGCGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAGTATAACCCTGCAATACGCCGACGCGGGAGAACTGGCGA
AAGCCGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCGCCCTTGAACAGTGGGTAGCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGCGTGAAATGGACGCTGACCGATGCGCAACAAGCTGGTGGCGTTG
GACAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCTACGGCGACAACGCATGTCGGTTTTAACATTGGGCGCATCAAC
GGACGTTTGCTGGATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCTAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAGGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCCGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGTGGCATTTTTACCCGTAAAAATAAATCGGGGCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  comE Haemophilus influenzae 86-028NP

36.927

100

0.391
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364