Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   SOI79_RS09295 Genome accession   NZ_CP139266
Coordinates   1941923..1942702 (+) Length   259 a.a.
NCBI ID   WP_032057248.1    Uniprot ID   A0A009QPU8
Organism   Acinetobacter pittii strain HUM14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1936923..1947702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI79_RS09280 (SOI79_09280) ponA 1937504..1940065 (-) 2562 WP_068976277.1 penicillin-binding protein PBP1a -
  SOI79_RS09285 (SOI79_09285) pilM 1940227..1941285 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  SOI79_RS09290 (SOI79_09290) pilN 1941285..1941926 (+) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  SOI79_RS09295 (SOI79_09295) pilO 1941923..1942702 (+) 780 WP_032057248.1 type 4a pilus biogenesis protein PilO Machinery gene
  SOI79_RS09300 (SOI79_09300) pilP 1942714..1943241 (+) 528 WP_005067086.1 pilus assembly protein PilP Machinery gene
  SOI79_RS09305 (SOI79_09305) pilQ 1943304..1945430 (+) 2127 WP_068976278.1 type IV pilus secretin PilQ Machinery gene
  SOI79_RS09310 (SOI79_09310) aroK 1945442..1946011 (+) 570 WP_032004887.1 shikimate kinase AroK -
  SOI79_RS09315 (SOI79_09315) aroB 1946040..1947122 (+) 1083 WP_068976279.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29449.53 Da        Isoelectric Point: 5.7456

>NTDB_id=908527 SOI79_RS09295 WP_032057248.1 1941923..1942702(+) (pilO) [Acinetobacter pittii strain HUM14]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGRSDIPVLNYSIKAKTYRYMGAVENQNNSDPQVINGESQI
NASDSRNAHIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=908527 SOI79_RS09295 WP_032057248.1 1941923..1942702(+) (pilO) [Acinetobacter pittii strain HUM14]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAGATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGCGCGGTTGAAAATCAAAATAATAGTGATCCTCAAGTGATAAATGGTGAAAGTCAGATA
AATGCCTCCGATAGCCGTAATGCACATATTCCTTCTATAAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009QPU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.286

86.486

0.772
  comO Acinetobacter baylyi ADP1

71.552

89.575

0.641