Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   TM2_RS11290 Genome accession   NZ_AP025262
Coordinates   2205532..2205825 (-) Length   97 a.a.
NCBI ID   WP_008342186.1    Uniprot ID   A0A5K1NAM4
Organism   Bacillus altitudinis strain TM2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2200532..2210825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TM2_RS11240 (TM2_21790) sinR 2200695..2201030 (+) 336 WP_017357978.1 transcriptional regulator SinR Regulator
  TM2_RS11245 (TM2_21800) tasA 2201098..2201898 (-) 801 WP_008342195.1 biofilm matrix protein TasA -
  TM2_RS11250 (TM2_21810) sipW 2201957..2202529 (-) 573 WP_017357979.1 signal peptidase I SipW -
  TM2_RS11255 (TM2_21820) tapA 2202580..2203107 (-) 528 WP_099496603.1 amyloid fiber anchoring/assembly protein TapA -
  TM2_RS11260 (TM2_21830) - 2203360..2203686 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  TM2_RS11265 (TM2_21840) - 2203721..2203915 (-) 195 WP_008342191.1 YqzE family protein -
  TM2_RS11270 (TM2_21850) - 2203976..2204359 (-) 384 WP_041091746.1 competence type IV pilus minor pilin ComGG -
  TM2_RS11275 (TM2_21860) comGF 2204356..2204853 (-) 498 WP_041091745.1 competence type IV pilus minor pilin ComGF -
  TM2_RS11280 (TM2_21870) comGE 2204795..2205109 (-) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  TM2_RS11285 (TM2_21880) comGD 2205093..2205539 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  TM2_RS11290 (TM2_21890) comGC 2205532..2205825 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  TM2_RS11295 (TM2_21900) comGB 2205842..2206882 (-) 1041 WP_017357986.1 competence type IV pilus assembly protein ComGB -
  TM2_RS11300 (TM2_21910) comGA 2206863..2207933 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  TM2_RS11305 (TM2_21930) - 2208399..2209268 (-) 870 WP_007501231.1 STAS domain-containing protein -
  TM2_RS11315 (TM2_21940) - 2209494..2209874 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  TM2_RS11320 (TM2_21950) - 2210087..2210329 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10728.62 Da        Isoelectric Point: 6.7081

>NTDB_id=90768 TM2_RS11290 WP_008342186.1 2205532..2205825(-) (comGC) [Bacillus altitudinis strain TM2]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQSIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIFIQNGDVKNE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=90768 TM2_RS11290 WP_008342186.1 2205532..2205825(-) (comGC) [Bacillus altitudinis strain TM2]
ATGAATGATAAAGGATTTACACTGTTAGAAATGTTAATTGTTATGCTTGTGATCTCCATTCTTTTATTGATCACCATTCC
AAATATCACTCGGCATCATCAAAGCATTCAAGCAAAGGGCTGTGAAGGGCTGAAATCAATGGTGAGTACACAGGTAATGG
CATATGAATTAGAGCATGACGGCAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGCTATGTCAAAAAAGGATTAACT
TGTCCAAATGGAAAAGCAATTTTCATTCAAAACGGCGATGTGAAAAATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5K1NAM4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

41.304

94.845

0.392
  comGC Staphylococcus aureus N315

41.304

94.845

0.392

Multiple sequence alignment