Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   TM2_RS11300 Genome accession   NZ_AP025262
Coordinates   2206863..2207933 (-) Length   356 a.a.
NCBI ID   WP_017357987.1    Uniprot ID   -
Organism   Bacillus altitudinis strain TM2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2201863..2212933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TM2_RS11250 (TM2_21810) sipW 2201957..2202529 (-) 573 WP_017357979.1 signal peptidase I SipW -
  TM2_RS11255 (TM2_21820) tapA 2202580..2203107 (-) 528 WP_099496603.1 amyloid fiber anchoring/assembly protein TapA -
  TM2_RS11260 (TM2_21830) - 2203360..2203686 (+) 327 WP_039166025.1 DUF3889 domain-containing protein -
  TM2_RS11265 (TM2_21840) - 2203721..2203915 (-) 195 WP_008342191.1 YqzE family protein -
  TM2_RS11270 (TM2_21850) - 2203976..2204359 (-) 384 WP_041091746.1 competence type IV pilus minor pilin ComGG -
  TM2_RS11275 (TM2_21860) comGF 2204356..2204853 (-) 498 WP_041091745.1 competence type IV pilus minor pilin ComGF -
  TM2_RS11280 (TM2_21870) comGE 2204795..2205109 (-) 315 WP_017357984.1 competence type IV pilus minor pilin ComGE -
  TM2_RS11285 (TM2_21880) comGD 2205093..2205539 (-) 447 WP_017357985.1 competence type IV pilus minor pilin ComGD -
  TM2_RS11290 (TM2_21890) comGC 2205532..2205825 (-) 294 WP_008342186.1 competence type IV pilus major pilin ComGC Machinery gene
  TM2_RS11295 (TM2_21900) comGB 2205842..2206882 (-) 1041 WP_017357986.1 competence type IV pilus assembly protein ComGB -
  TM2_RS11300 (TM2_21910) comGA 2206863..2207933 (-) 1071 WP_017357987.1 competence type IV pilus ATPase ComGA Machinery gene
  TM2_RS11305 (TM2_21930) - 2208399..2209268 (-) 870 WP_007501231.1 STAS domain-containing protein -
  TM2_RS11315 (TM2_21940) - 2209494..2209874 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  TM2_RS11320 (TM2_21950) - 2210087..2210329 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  TM2_RS11325 (TM2_21960) - 2210378..2211016 (-) 639 WP_017357989.1 MBL fold metallo-hydrolase -
  TM2_RS11330 (TM2_21970) - 2211177..2211350 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  TM2_RS11335 (TM2_21980) - 2211574..2211888 (-) 315 WP_008342314.1 MTH1187 family thiamine-binding protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40339.70 Da        Isoelectric Point: 9.0449

>NTDB_id=90769 TM2_RS11300 WP_017357987.1 2206863..2207933(-) (comGA) [Bacillus altitudinis strain TM2]
MYGIEYLGQELLEEACRMRASDVHIVPREKEASVSFRVDADLIQQRIIDKKSGERLIAHFKFLSSMDIGERRRPQNGSLA
VMLRRGQVFVRLSTLPTVNDESLVIRILPQDHVPKTKYLSLFPKASARLLSFLNHSHGLILFTGPTNSGKTTTLYSLIQF
AKKHFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVMSTLH
AKNAKGALYRMLEFGVTMNELEQTMVAIAAQRLIELACPFCGETCELYCKLNRPVRRTNVFELLYGKELGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=90769 TM2_RS11300 WP_017357987.1 2206863..2207933(-) (comGA) [Bacillus altitudinis strain TM2]
TTGTATGGTATTGAATATTTGGGGCAAGAGCTGCTAGAAGAGGCGTGCCGCATGAGAGCATCTGATGTGCATATTGTTCC
GAGAGAAAAGGAAGCTTCAGTCTCTTTTCGTGTAGACGCTGACCTCATTCAGCAGCGGATCATTGACAAAAAGAGCGGAG
AGCGGCTCATTGCTCATTTCAAATTTTTATCTTCTATGGATATTGGTGAAAGAAGGAGACCACAAAATGGATCATTGGCT
GTGATGTTAAGACGCGGTCAAGTGTTTGTTCGGCTTTCTACATTACCGACTGTAAATGATGAAAGTTTAGTGATTCGGAT
TTTACCGCAGGATCATGTGCCGAAAACAAAGTATTTGTCACTGTTCCCGAAGGCTTCAGCGAGATTATTATCGTTTCTGA
ATCATTCGCATGGGCTCATTTTATTTACTGGACCAACAAATTCAGGGAAAACGACAACACTTTATTCATTGATCCAGTTT
GCCAAAAAACATTTTAATCGAAATATTATTACCCTTGAAGATCCTGTGGAAACAAGAAATGAAGAAGTGCTGCAGGTTCA
AGTAAATGAAAAAGCTGGTATCACGTATGCGGCAGGTTTACGCGCTATTTTAAGACATGATCCAGATATGATTGTGCTTG
GAGAAATAAGAGATGCGGAAACAGCGAGAACAGCTATTAGAGCAGCTTTGACAGGTCATTTAGTCATGAGCACACTGCAC
GCAAAGAATGCAAAAGGAGCTCTTTATCGAATGCTCGAATTTGGTGTGACGATGAATGAACTCGAACAAACAATGGTTGC
CATTGCTGCTCAGCGGTTAATCGAACTCGCATGTCCATTTTGCGGCGAAACGTGTGAGCTTTATTGCAAACTAAATCGGC
CAGTCAGACGAACAAATGTATTTGAATTGCTTTACGGAAAGGAGCTTGGTGAGTGTATCAAAGAGGCAAAAGGTGAATAC
GCTCATTCGTCATACGAAACACTGCAAAGATTAATTCGGAAAGGAGTGGCACTCGGCTATTTATCAAAAAGCACATATCA
TCGATGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

65.254

99.438

0.649

Multiple sequence alignment