Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SIS06_RS14010 Genome accession   NZ_CP139188
Coordinates   2643439..2644056 (-) Length   205 a.a.
NCBI ID   WP_232567367.1    Uniprot ID   -
Organism   Bacillus subtilis strain H17-16     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2638439..2649056
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIS06_RS13980 (SIS06_13980) rpsT 2638741..2639007 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  SIS06_RS13985 (SIS06_13985) holA 2639022..2640065 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  SIS06_RS13990 (SIS06_13990) - 2640105..2640218 (-) 114 WP_134975484.1 hypothetical protein -
  SIS06_RS13995 (SIS06_13995) yqzM 2640295..2640429 (+) 135 WP_003229983.1 YqzM family protein -
  SIS06_RS14000 (SIS06_14000) comEC 2640469..2642799 (-) 2331 WP_172861318.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SIS06_RS14005 (SIS06_14005) comEB 2642803..2643372 (-) 570 WP_003229978.1 ComE operon protein 2 -
  SIS06_RS14010 (SIS06_14010) comEA 2643439..2644056 (-) 618 WP_232567367.1 competence protein ComEA Machinery gene
  SIS06_RS14015 (SIS06_14015) comER 2644140..2644961 (+) 822 WP_077671455.1 late competence protein ComER -
  SIS06_RS14020 (SIS06_14020) yqeM 2645027..2645770 (-) 744 WP_072174289.1 class I SAM-dependent DNA methyltransferase -
  SIS06_RS14025 (SIS06_14025) rsfS 2645767..2646123 (-) 357 WP_014477391.1 ribosome silencing factor -
  SIS06_RS14030 (SIS06_14030) yqeK 2646141..2646701 (-) 561 WP_283933574.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SIS06_RS14035 (SIS06_14035) nadD 2646691..2647260 (-) 570 WP_283933575.1 nicotinate-nucleotide adenylyltransferase -
  SIS06_RS14040 (SIS06_14040) yhbY 2647272..2647562 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  SIS06_RS14045 (SIS06_14045) aroE 2647556..2648398 (-) 843 WP_283933576.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21813.46 Da        Isoelectric Point: 4.6388

>NTDB_id=907644 SIS06_RS14010 WP_232567367.1 2643439..2644056(-) (comEA) [Bacillus subtilis strain H17-16]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQDISGV
GPSKAEAIVAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=907644 SIS06_RS14010 WP_232567367.1 2643439..2644056(-) (comEA) [Bacillus subtilis strain H17-16]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTCTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAACAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAATTACAAGACATCTCGGGGGTG
GGGCCATCCAAAGCTGAAGCTATTGTTGCATACCGGGAAGAAAACGGACGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

99.024

100

0.99
  comEA Staphylococcus aureus MW2

37.727

100

0.405
  comEA Staphylococcus aureus N315

36.818

100

0.395