Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   SH592_RS12255 Genome accession   NZ_CP139053
Coordinates   2549556..2550170 (-) Length   204 a.a.
NCBI ID   WP_015240245.1    Uniprot ID   -
Organism   Bacillus velezensis strain Y-4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544556..2555170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SH592_RS12225 (SH592_12225) rpsT 2544859..2545125 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  SH592_RS12230 (SH592_12230) holA 2545142..2546183 (-) 1042 Protein_2361 DNA polymerase III subunit delta -
  SH592_RS12235 (SH592_12235) - 2546223..2546374 (-) 152 Protein_2362 hypothetical protein -
  SH592_RS12240 (SH592_12240) - 2546415..2546549 (+) 135 WP_003152870.1 YqzM family protein -
  SH592_RS12245 (SH592_12245) comEC 2546568..2548919 (-) 2352 WP_039254415.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SH592_RS12250 (SH592_12250) - 2548920..2549489 (-) 570 WP_003152868.1 ComE operon protein 2 -
  SH592_RS12255 (SH592_12255) comEA 2549556..2550170 (-) 615 WP_015240245.1 helix-hairpin-helix domain-containing protein Machinery gene
  SH592_RS12260 (SH592_12260) comER 2550229..2551050 (+) 822 WP_039254409.1 late competence protein ComER -
  SH592_RS12265 (SH592_12265) - 2551119..2551856 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  SH592_RS12270 (SH592_12270) rsfS 2551853..2552209 (-) 357 WP_007408260.1 ribosome silencing factor -
  SH592_RS12275 (SH592_12275) yqeK 2552227..2552787 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  SH592_RS12280 (SH592_12280) - 2552777..2553346 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  SH592_RS12285 (SH592_12285) yhbY 2553357..2553647 (-) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  SH592_RS12290 (SH592_12290) aroE 2553641..2554483 (-) 843 WP_039254404.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22209.32 Da        Isoelectric Point: 5.5211

>NTDB_id=907040 SH592_RS12255 WP_015240245.1 2549556..2550170(-) (comEA) [Bacillus velezensis strain Y-4]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=907040 SH592_RS12255 WP_015240245.1 2549556..2550170(-) (comEA) [Bacillus velezensis strain Y-4]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363