NTDB

Overview

Name	comGF	Type	Machinery gene
Locus tag	SAKRST72_RS07375	Genome accession	NZ_CP138568
Coordinates	1554324..1554821 (-)	Length	165 a.a.
NCBI ID	WP_020978197.1	Uniprot ID	-
Organism	Staphylococcus aureus strain SAWL001
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1549324..1559821

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SAKRST72_RS07350 (SAKRST72_07350)	gcvPB	1549486..1550958 (-)	1473	WP_000202188.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
SAKRST72_RS07355 (SAKRST72_07355)	gcvPA	1550951..1552297 (-)	1347	WP_000019681.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
SAKRST72_RS07360 (SAKRST72_07360)	gcvT	1552317..1553408 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
SAKRST72_RS07365 (SAKRST72_07365)	-	1553567..1554091 (-)	525	WP_001015121.1	shikimate kinase	-
SAKRST72_RS07370 (SAKRST72_07370)	-	1554081..1554227 (-)	147	WP_001789879.1	hypothetical protein	-
SAKRST72_RS07375 (SAKRST72_07375)	comGF	1554324..1554821 (-)	498	WP_020978197.1	competence type IV pilus minor pilin ComGF	Machinery gene
SAKRST72_RS07380 (SAKRST72_07380)	comGE	1554739..1555038 (-)	300	WP_000844410.1	hypothetical protein	Machinery gene
SAKRST72_RS07385 (SAKRST72_07385)	comGD	1555025..1555471 (-)	447	WP_001788899.1	competence type IV pilus minor pilin ComGD	Machinery gene
SAKRST72_RS07390 (SAKRST72_07390)	comGC	1555449..1555760 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
SAKRST72_RS07395 (SAKRST72_07395)	comGB	1555774..1556844 (-)	1071	WP_000776401.1	competence type IV pilus assembly protein ComGB	Machinery gene
SAKRST72_RS07400 (SAKRST72_07400)	comGA	1556816..1557790 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
SAKRST72_RS07405 (SAKRST72_07405)	-	1557842..1558465 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
SAKRST72_RS07410 (SAKRST72_07410)	-	1558462..1558791 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
SAKRST72_RS07415 (SAKRST72_07415)	-	1558791..1559777 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

Download Length: 165 a.a. Molecular weight: 19158.95 Da Isoelectric Point: 10.1964

                                >NTDB_id=903524 SAKRST72_RS07375 WP_020978197.1 1554324..1554821(-) (comGF) [Staphylococcus aureus strain SAWL001]

MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI

LEDFKGVDKNDIEIRQQRIILQKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT

KTIYV

Nucleotide

Download Length: 498 bp

                                >NTDB_id=903524 SAKRST72_RS07375 WP_020978197.1 1554324..1554821(-) (comGF) [Staphylococcus aureus strain SAWL001]

ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT

TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT

TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT

CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACAGAAAGGTGAAGAAAT

GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA

CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT

AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF	Staphylococcus aureus N315	98.788	100	0.988
comGF	Staphylococcus aureus MW2	96.364	100	0.964

Detailed information NTG0903524