Detailed information NTG0902539

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SD196_RS15505
• Genome accession: NZ_CP138377
• Coordinates: 3381133..3382356 (-)
• Length: 407 a.a.
• NCBI ID: WP_275611600.1
• Uniprot ID: -
• Organism: Pseudomonas sp. W03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3376133..3387356

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SD196_RS15490 (SD196_15465)	-	3376997..3377815 (+)	819	WP_416310383.1	helix-turn-helix domain-containing protein	-
SD196_RS15495 (SD196_15470)	-	3377936..3378820 (-)	885	WP_416310384.1	DMT family transporter	-
SD196_RS15500 (SD196_15475)	-	3379303..3380553 (+)	1251	WP_343573597.1	OprD family porin	-
SD196_RS15505 (SD196_15480)	pilU	3381133..3382356 (-)	1224	WP_275611600.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SD196_RS15510 (SD196_15485)	-	3382543..3382821 (+)	279	WP_152223913.1	peptidylprolyl isomerase	-
SD196_RS15515 (SD196_15490)	-	3382829..3383194 (+)	366	WP_275611599.1	MmcQ/YjbR family DNA-binding protein	-
SD196_RS15520 (SD196_15495)	-	3383191..3383745 (+)	555	WP_416310385.1	putative glycolipid-binding domain-containing protein	-
SD196_RS15525 (SD196_15500)	-	3384087..3385268 (+)	1182	WP_370601523.1	MFS transporter	-
SD196_RS15530 (SD196_15505)	-	3385535..3386812 (+)	1278	WP_416310386.1	OprD family porin	-
SD196_RS15535 (SD196_15510)	-	3386871..3387158 (-)	288	WP_275611595.1	hypothetical protein	-

Sequence

Protein

• Length: 407 a.a.
• Molecular weight: 44429.64 Da
• Isoelectric Point: 5.9218

>NTDB_id=902539 SD196_RS15505 WP_275611600.1 3381133..3382356(-) (pilU) [Pseudomonas sp. W03]
MDIQSMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSNEPLKAGDVARIGYGLMDEEQRAEFERELEMNLAISVQGVGR
FRINLFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDLIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLYKDNPAMVTNSAAATAASPAPAPAAAPAPAADSSSWGLELALEEIEAEDA
EGKPAGQ

Nucleotide

• Length: 1224 bp

>NTDB_id=902539 SD196_RS15505 WP_275611600.1 3381133..3382356(-) (pilU) [Pseudomonas sp. W03]
ATGGATATCCAGTCGATGCTGAAGATCCTGGCCACCCAGGATGGTTCCGACCTCTACCTGTCCACCGGCGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAACGAGCCGCTCAAGGCGGGCGACGTGGCGCGCATCGGCTACGGCC
TGATGGACGAGGAACAGCGCGCCGAGTTCGAACGGGAACTGGAGATGAACCTGGCCATCTCGGTGCAGGGTGTCGGCCGC
TTCCGCATCAACCTGTTCAAGCAGCGCAACGAAGTGTCCATCGTGGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
GGACCTGAAGCTGCCGGAAGTGCTGCTCAAGGTGGTCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGTGAGGTGGGCGTGGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAACATG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGTCGCGCAGCGGTGGAAGTGCTGCTGGGCACCGCCACCATCAGCGACCTGATCA
AGCGCGGCGACTTCAGCTCGATCAAGGAAATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAGGGGGCGATCAGCGAAGAAGAAGCGGTGAAGAACGCCGATTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTGTACAAGGACAACCCGGCCATGGTGACCAACTCCGCCGCAGCCACTGCCGCCAGCCCCGCGCCGGCACCCG
CCGCAGCGCCTGCCCCGGCAGCGGACTCCTCCAGCTGGGGGCTGGAACTGGCGCTGGAAGAGATCGAGGCCGAAGACGCG
GAAGGCAAGCCGGCTGGGCAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.98	86.241	0.491
pilU	Acinetobacter baylyi ADP1	53.693	86.486	0.464
pilU	Vibrio cholerae strain A1552	52.286	85.995	0.45
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.896	94.84	0.378