Detailed information NTG0901811

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SD209_RS29460
• Genome accession: NZ_CP138326
• Coordinates: 6206398..6207546 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain JBPA
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6201398..6212546

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SD209_RS29435 (SD209_29435)	-	6202549..6202845 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
SD209_RS29440 (SD209_29440)	-	6202842..6203435 (-)	594	WP_003084544.1	YggT family protein	-
SD209_RS29445 (SD209_29445)	proC	6203446..6204267 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
SD209_RS29450 (SD209_29450)	-	6204279..6204971 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
SD209_RS29455 (SD209_29455)	pilT	6205186..6206220 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
SD209_RS29460 (SD209_29460)	pilU	6206398..6207546 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
SD209_RS29465 (SD209_29465)	czcD	6207554..6208453 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
SD209_RS29470 (SD209_29470)	-	6208607..6209011 (+)	405	WP_003084560.1	NINE protein	-
SD209_RS29475 (SD209_29475)	-	6209254..6210627 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
SD209_RS29480 (SD209_29480)	-	6210624..6211808 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=901811 SD209_RS29460 WP_003100959.1 6206398..6207546(+) (pilU) [Pseudomonas aeruginosa strain JBPA]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=901811 SD209_RS29460 WP_003100959.1 6206398..6207546(+) (pilU) [Pseudomonas aeruginosa strain JBPA]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382