Detailed information NTG0901763

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: SD207_RS13235
• Genome accession: NZ_CP138324
• Coordinates: 2809216..2810334 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain JBAB
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2804216..2815334

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SD207_RS13225 (SD207_13225)	-	2807331..2808023 (-)	693	WP_001108508.1	YggS family pyridoxal phosphate-dependent enzyme	-
SD207_RS13230 (SD207_13230)	pilT	2808151..2809188 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
SD207_RS13235 (SD207_13235)	pilU	2809216..2810334 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
SD207_RS13240 (SD207_13240)	fur	2810404..2810841 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
SD207_RS13245 (SD207_13245)	-	2810953..2811351 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
SD207_RS13250 (SD207_13250)	-	2811406..2811729 (-)	324	WP_000442585.1	RnfH family protein	-
SD207_RS13255 (SD207_13255)	-	2811729..2812796 (-)	1068	WP_002084046.1	hypothetical protein	-
SD207_RS13260 (SD207_13260)	-	2812949..2813404 (-)	456	WP_000782976.1	bacteriohemerythrin	-
SD207_RS13265 (SD207_13265)	-	2813657..2814505 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=901763 SD207_RS13235 WP_000347036.1 2809216..2810334(+) (pilU) [Acinetobacter baumannii strain JBAB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=901763 SD207_RS13235 WP_000347036.1 2809216..2810334(+) (pilU) [Acinetobacter baumannii strain JBAB]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCACCAGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTTGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGCCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392