Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   TthTMY_RS02935 Genome accession   NZ_AP025158
Coordinates   510860..511483 (-) Length   207 a.a.
NCBI ID   WP_223903394.1    Uniprot ID   -
Organism   Thermus thermophilus strain TMY     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 505860..516483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TthTMY_RS02915 (TthTMY_05730) aroC 505934..507085 (-) 1152 WP_096412258.1 chorismate synthase -
  TthTMY_RS02920 (TthTMY_05740) pilQ 507144..509417 (-) 2274 WP_096412261.1 secretin N-terminal domain-containing protein Machinery gene
  TthTMY_RS02925 (TthTMY_05750) pilW 509414..510292 (-) 879 WP_096412264.1 competence protein Machinery gene
  TthTMY_RS02930 (TthTMY_05760) pilO 510289..510870 (-) 582 WP_096412267.1 type 4a pilus biogenesis protein PilO Machinery gene
  TthTMY_RS02935 (TthTMY_05770) pilN 510860..511483 (-) 624 WP_223903394.1 flagellar protein FliT Machinery gene
  TthTMY_RS02940 (TthTMY_05780) pilM 511476..512609 (-) 1134 WP_096412270.1 type IV pilus assembly protein PilM Machinery gene
  TthTMY_RS02945 (TthTMY_05790) - 512744..513748 (-) 1005 WP_096412273.1 homoisocitrate dehydrogenase -
  TthTMY_RS02950 (TthTMY_05800) - 513803..514294 (-) 492 WP_096413136.1 DUF5317 domain-containing protein -
  TthTMY_RS02955 (TthTMY_05820) - 514345..515586 (-) 1242 WP_096412276.1 HD-GYP domain-containing protein -
  TthTMY_RS02960 (TthTMY_05830) - 515585..516430 (+) 846 WP_096410485.1 transposase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23083.56 Da        Isoelectric Point: 9.7530

>NTDB_id=90167 TthTMY_RS02935 WP_223903394.1 510860..511483(-) (pilN) [Thermus thermophilus strain TMY]
MIRLNLLPKNLRRRVEPGWWRLLAFLFALVVLGVLGLVHYTAYTELSLAKAERDQLQAEVEALRPFIAELGRLQEERKAL
EALLAIREGLEKNAVPWSQYLAAFINQIPRAGGRLEVALRSVSARALSEEEAARVAQEGTYDGKRIRVEFALQGEALSRE
ALVRFIRAFETSPRFGIEFQGASLDEGRGLYTFSARVGVTGGESGAR

Nucleotide


Download         Length: 624 bp        

>NTDB_id=90167 TthTMY_RS02935 WP_223903394.1 510860..511483(-) (pilN) [Thermus thermophilus strain TMY]
TTGATTAGGCTGAACCTCCTCCCCAAGAACCTCCGGCGCCGGGTGGAACCGGGCTGGTGGCGCCTTCTCGCCTTCCTCTT
CGCCCTGGTCGTCCTCGGGGTCTTGGGGCTCGTTCACTACACCGCCTACACCGAGCTTTCCCTGGCCAAGGCGGAGCGGG
ACCAGCTCCAGGCGGAGGTGGAGGCCCTGAGGCCCTTCATCGCCGAGCTCGGCCGCCTGCAGGAGGAGCGTAAGGCCCTC
GAGGCCCTCCTCGCCATCCGGGAAGGCCTGGAGAAAAACGCCGTGCCCTGGTCCCAGTACCTGGCCGCCTTCATCAACCA
GATCCCCAGGGCGGGGGGGCGCCTCGAGGTGGCCCTCCGCTCGGTGAGCGCCCGCGCCCTCTCGGAAGAGGAGGCCGCCC
GCGTGGCCCAGGAGGGCACCTACGACGGCAAGCGGATCCGGGTGGAGTTCGCCCTCCAGGGGGAGGCCCTGAGCCGGGAG
GCCTTGGTCCGGTTCATCCGGGCCTTTGAGACCTCTCCCCGGTTCGGCATTGAGTTCCAAGGGGCCTCCCTGGACGAGGG
CCGGGGGCTTTACACCTTCAGCGCCCGCGTGGGCGTGACGGGGGGTGAAAGCGGTGCTCGCTAG

Domains

Predicted by InterproScan.

PilN biogenesis protein dimerization domain (PilN_bio_d)

(97-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Thermus thermophilus HB27

98.551

100

0.986

Multiple sequence alignment