Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TthTMY_RS02940 Genome accession   NZ_AP025158
Coordinates   511476..512609 (-) Length   377 a.a.
NCBI ID   WP_096412270.1    Uniprot ID   -
Organism   Thermus thermophilus strain TMY     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506476..517609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TthTMY_RS02920 (TthTMY_05740) pilQ 507144..509417 (-) 2274 WP_096412261.1 secretin N-terminal domain-containing protein Machinery gene
  TthTMY_RS02925 (TthTMY_05750) pilW 509414..510292 (-) 879 WP_096412264.1 competence protein Machinery gene
  TthTMY_RS02930 (TthTMY_05760) pilO 510289..510870 (-) 582 WP_096412267.1 type 4a pilus biogenesis protein PilO Machinery gene
  TthTMY_RS02935 (TthTMY_05770) pilN 510860..511483 (-) 624 WP_223903394.1 flagellar protein FliT Machinery gene
  TthTMY_RS02940 (TthTMY_05780) pilM 511476..512609 (-) 1134 WP_096412270.1 type IV pilus assembly protein PilM Machinery gene
  TthTMY_RS02945 (TthTMY_05790) - 512744..513748 (-) 1005 WP_096412273.1 homoisocitrate dehydrogenase -
  TthTMY_RS02950 (TthTMY_05800) - 513803..514294 (-) 492 WP_096413136.1 DUF5317 domain-containing protein -
  TthTMY_RS02955 (TthTMY_05820) - 514345..515586 (-) 1242 WP_096412276.1 HD-GYP domain-containing protein -
  TthTMY_RS02960 (TthTMY_05830) - 515585..516430 (+) 846 WP_096410485.1 transposase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41419.04 Da        Isoelectric Point: 4.4419

>NTDB_id=90168 TthTMY_RS02940 WP_096412270.1 511476..512609(-) (pilM) [Thermus thermophilus strain TMY]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLVEGMVAEPTALAQEIKELLLEARTRKRYVI
TALSNLSVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEARLAEEPHRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLA
AEEVKRTYGMATIPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEASPEVGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVAVDPKRFEAEQLQEMGPEFAVALGLALRGVEPLD

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=90168 TthTMY_RS02940 WP_096412270.1 511476..512609(-) (pilM) [Thermus thermophilus strain TMY]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGGTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAAGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGGTGGAAGGGA
TGGTGGCCGAGCCTACCGCGTTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGATC
ACCGCCCTCTCCAACCTGTCGGTGATCCTCCGCCCCATCCAGGTCCCCAAGATGCCCCTCAAGGAGATGGAAGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATTCCCTTCCCCATTGACGAAGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCCTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTTTACCCTCTGGAGGCCAGGCTTGCGGA
GGAACCCCACCGGGTCTTCCTCGTCCTGGACATCGGGGCCGAGAGCACGAGCCTCGTCCTGCTCCGGGGGGACAAGCCCC
TGGCAGTGCGCGTCCTCACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCCCGAAGCTTCAACCTGGACCTCCTCGCC
GCCGAAGAGGTGAAGCGGACCTACGGGATGGCCACCATCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
ACGGGAGCGCTACAGCCCGGGGCGCATCTACGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTGGAGTTCTTCCGCATCCAGCTGGAGGAGGCCTCGCCCGAGGTGGGCTACCTCCTGGGCGGGGGGAGCAAGCTCAGG
GGGCTTGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTTGAGCCCGTGAACCCCTGGGAGGCGGTGGCGGTGGACCC
CAAGCGCTTTGAGGCGGAGCAACTCCAGGAGATGGGGCCGGAGTTCGCCGTGGCCCTGGGCTTGGCGTTGAGGGGGGTGG
AGCCCCTTGATTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-372)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Thermus thermophilus HB27

97.613

100

0.976

Multiple sequence alignment