Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SBP02_RS19325 Genome accession   NZ_CP137892
Coordinates   4180714..4181859 (-) Length   381 a.a.
NCBI ID   WP_318644020.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain HNIBRBA3361     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4175714..4186859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBP02_RS19295 (SBP02_19295) - 4176314..4176883 (+) 570 WP_318644014.1 YqgE/AlgH family protein -
  SBP02_RS19300 (SBP02_19300) ruvX 4176883..4177326 (+) 444 WP_318644015.1 Holliday junction resolvase RuvX -
  SBP02_RS19305 (SBP02_19305) pyrR 4177356..4177862 (+) 507 WP_318644016.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  SBP02_RS19310 (SBP02_19310) - 4177874..4178878 (+) 1005 WP_318644017.1 aspartate carbamoyltransferase catalytic subunit -
  SBP02_RS19315 (SBP02_19315) - 4178878..4180149 (+) 1272 WP_318644018.1 dihydroorotase -
  SBP02_RS19320 (SBP02_19320) - 4180208..4180609 (-) 402 WP_318644019.1 TM2 domain-containing protein -
  SBP02_RS19325 (SBP02_19325) pilU 4180714..4181859 (-) 1146 WP_318644020.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBP02_RS19330 (SBP02_19330) pilT 4181971..4183005 (-) 1035 WP_318644021.1 type IV pilus twitching motility protein PilT Machinery gene
  SBP02_RS19335 (SBP02_19335) - 4183079..4183771 (+) 693 WP_318644022.1 YggS family pyridoxal phosphate-dependent enzyme -
  SBP02_RS19340 (SBP02_19340) proC 4183786..4184604 (+) 819 WP_318644023.1 pyrroline-5-carboxylate reductase -
  SBP02_RS19345 (SBP02_19345) - 4184615..4185205 (+) 591 WP_318644024.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42491.66 Da        Isoelectric Point: 6.5932

>NTDB_id=901253 SBP02_RS19325 WP_318644020.1 4180714..4181859(-) (pilU) [Pseudomonas benzenivorans strain HNIBRBA3361]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVTKNPMSPEQTRETVYAVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDDLKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQNCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKGLMKRSTEQGMQTFDQA
LYALYSQGEITYEDALLYADSANDLRLMIKLGSESDGDHLAQLSEGLSLETTPEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=901253 SBP02_RS19325 WP_318644020.1 4180714..4181859(-) (pilU) [Pseudomonas benzenivorans strain HNIBRBA3361]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTGGAGAAGGGCGGCTCGGACCTGTTCATCACCGCCGGGGTGCCACCGTC
CATGAAGGTCAACGGCAAGATCATGCCGGTCACCAAGAACCCGATGTCGCCGGAGCAGACCCGGGAAACCGTGTACGCGG
TGATGAACGAGCAGCAGCGCCGGGAGTTCGCCGAGAATCACGAATGCAACTTCGCCATCAGCGCCCGCGGCGTGGGGCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGCGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCCTGGA
TGACCTCAAGCTGCCGGAGGTGCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CCGGCAAGTCGACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAACTGCATCGTCACCCAGCGCGAGGTCGGGATCGACACCGAGTCCTACGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGGTGCGCACCCGCGAGACCATGGATCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCCGCCGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCGGATGGCAAGGGGCGGCGGGCGGTGATCGAGGTGTTGATCAACACCCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGGTCTGATGAAGCGCTCCACCGAACAGGGCATGCAGACCTTCGACCAGGCG
CTCTATGCCCTCTACAGCCAGGGCGAGATCACCTACGAGGATGCGCTGCTGTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGCTCGGAAAGCGACGGCGATCACCTGGCCCAGCTGAGCGAGGGCCTGTCCCTGGAGACCACACCGG
AAGACCCGGGGCGGCGTTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.601

100

0.916
  pilU Acinetobacter baylyi ADP1

63.866

93.701

0.598
  pilU Vibrio cholerae strain A1552

59.429

91.864

0.546
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.155

88.189

0.381