Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SBP02_RS10025 Genome accession   NZ_CP137892
Coordinates   2199846..2201057 (+) Length   403 a.a.
NCBI ID   WP_318646242.1    Uniprot ID   -
Organism   Pseudomonas benzenivorans strain HNIBRBA3361     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2194846..2206057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SBP02_RS10005 (SBP02_10005) - 2194959..2197226 (-) 2268 WP_318646238.1 ATP-dependent DNA helicase -
  SBP02_RS10010 (SBP02_10010) - 2197223..2198863 (-) 1641 WP_318646239.1 VRR-NUC domain-containing protein -
  SBP02_RS10015 (SBP02_10015) - 2199003..2199431 (+) 429 WP_318646240.1 group II truncated hemoglobin -
  SBP02_RS10020 (SBP02_10020) - 2199419..2199697 (-) 279 WP_318646241.1 peptidylprolyl isomerase -
  SBP02_RS10025 (SBP02_10025) pilU 2199846..2201057 (+) 1212 WP_318646242.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SBP02_RS10030 (SBP02_10030) - 2201209..2201811 (+) 603 WP_318646243.1 DJ-1/PfpI family protein -
  SBP02_RS10035 (SBP02_10035) - 2201867..2202181 (-) 315 WP_318646244.1 cupin domain-containing protein -
  SBP02_RS10040 (SBP02_10040) - 2202248..2202886 (-) 639 WP_318646245.1 glutathione S-transferase -
  SBP02_RS10045 (SBP02_10045) - 2203091..2203402 (-) 312 WP_318646246.1 chorismate mutase -
  SBP02_RS10050 (SBP02_10050) - 2203443..2203937 (-) 495 WP_318646247.1 DUF3291 domain-containing protein -
  SBP02_RS10055 (SBP02_10055) - 2203987..2204838 (-) 852 WP_318646248.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 44507.80 Da        Isoelectric Point: 6.0824

>NTDB_id=901235 SBP02_RS10025 WP_318646242.1 2199846..2201057(+) (pilU) [Pseudomonas benzenivorans strain HNIBRBA3361]
MDLHSLLKILASQDGSDLYLSTGAPPCAKFNGVLKALGQEPLKSGDVAQVAESIMDSAQREEFERELEMNLAISLAGIGR
FRINIFKQRNEVSIVARNIKMEIPKFEDLKLPEVLLKTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTADGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLYRDNPVTPSPTPVAEKAAATPAAASEKGDWGLELKLEAIEEDSPPEDPGR
TGI

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=901235 SBP02_RS10025 WP_318646242.1 2199846..2201057(+) (pilU) [Pseudomonas benzenivorans strain HNIBRBA3361]
ATGGACCTTCATTCGCTGCTGAAAATCCTGGCCAGCCAGGACGGTTCCGATCTCTACCTGTCCACCGGTGCGCCACCCTG
TGCGAAGTTCAACGGCGTGCTCAAGGCCCTCGGTCAGGAGCCGTTGAAGTCCGGCGATGTGGCGCAGGTGGCCGAGTCGA
TCATGGACAGCGCGCAGCGCGAGGAATTCGAGCGCGAACTGGAGATGAACCTGGCGATTTCCCTGGCCGGGATCGGCCGC
TTCCGCATCAATATCTTCAAGCAGCGCAACGAAGTCTCGATCGTCGCGCGCAACATCAAGATGGAGATCCCCAAGTTCGA
GGACCTCAAGCTGCCCGAGGTGTTGCTCAAGACCGTGATGGAGAAGCGCGGCCTGGTACTGTTCGTCGGCGGCACCGGTT
CGGGCAAGTCCACCTCCCTGGCGGCGTTGATCGACTACCGCAACCGCAACAGCGGCGGTCATATCATCACCATCGAGGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGGGTCGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATCTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTCAAGACCGCCGACGGCAAGCGCCGCGCCGCGGTGGAGGTGATGCTCGGCACGCCGACGGTGCGCGACCTGATCA
AACGCAACGAGTTTTCCGAGCTCAAGGAGATCATGGAGAAGTCGAAGAACCTCGGCATGCAAACCTTCGACCAGGCGCTG
ATCGACCTGGTCCACGAGGGCGCCATCGACGAGGAAGAGGCGGTGAAGAACGCCGATTCGGCGAACAACGTGCGCCTCAA
GCTCAAGCTGTACCGGGATAACCCGGTCACGCCGAGCCCGACCCCCGTGGCCGAGAAGGCCGCTGCGACGCCTGCCGCGG
CGAGCGAGAAGGGCGACTGGGGGCTGGAGCTGAAACTGGAGGCGATCGAGGAAGATTCGCCGCCCGAGGACCCGGGGCGG
ACGGGGATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

87.097

0.494
  pilU Vibrio cholerae strain A1552

52.125

87.593

0.457
  pilU Acinetobacter baylyi ADP1

52.273

87.345

0.457
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.541

97.27

0.385