Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R6I15_RS26500 Genome accession   NZ_CP137716
Coordinates   5727756..5728820 (-) Length   354 a.a.
NCBI ID   WP_003110888.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa isolate FELIX_MS463     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 5684795..5731473 5727756..5728820 within 0


Gene organization within MGE regions


Location: 5684795..5731473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6I15_RS26230 (R6I15_26230) pilQ 5684795..5686939 (-) 2145 WP_003114575.1 type 4a pilus secretin PilQ Machinery gene
  R6I15_RS26235 (R6I15_26235) - 5686993..5687103 (-) 111 Protein_5178 pilus assembly protein PilP -
  R6I15_RS26240 (R6I15_26240) - 5687184..5687408 (-) 225 WP_003121473.1 hypothetical protein -
  R6I15_RS26245 (R6I15_26245) - 5687482..5687769 (-) 288 WP_010791802.1 hypothetical protein -
  R6I15_RS26250 (R6I15_26250) - 5687766..5688914 (-) 1149 WP_015648033.1 DUF2793 domain-containing protein -
  R6I15_RS26255 (R6I15_26255) - 5688911..5691121 (-) 2211 WP_010791804.1 hypothetical protein -
  R6I15_RS26260 (R6I15_26260) - 5691335..5691565 (-) 231 WP_010791805.1 hypothetical protein -
  R6I15_RS26265 (R6I15_26265) - 5691575..5692393 (-) 819 WP_010791806.1 phage BR0599 family protein -
  R6I15_RS26270 (R6I15_26270) - 5692380..5694086 (-) 1707 WP_010791807.1 hypothetical protein -
  R6I15_RS26275 (R6I15_26275) - 5694086..5695009 (-) 924 WP_010791808.1 hypothetical protein -
  R6I15_RS26280 (R6I15_26280) - 5695012..5695971 (-) 960 WP_010791809.1 hypothetical protein -
  R6I15_RS26285 (R6I15_26285) - 5695971..5699603 (-) 3633 WP_015649429.1 tape measure protein -
  R6I15_RS26290 (R6I15_26290) - 5699857..5700339 (-) 483 WP_010791811.1 DUF6631 family protein -
  R6I15_RS26295 (R6I15_26295) - 5700342..5701082 (-) 741 WP_010791812.1 hypothetical protein -
  R6I15_RS26300 (R6I15_26300) - 5701089..5701292 (-) 204 WP_010791813.1 hypothetical protein -
  R6I15_RS26305 (R6I15_26305) - 5701289..5701741 (-) 453 WP_010791814.1 hypothetical protein -
  R6I15_RS26310 (R6I15_26310) - 5701738..5702253 (-) 516 WP_003121492.1 gp436 family protein -
  R6I15_RS26315 (R6I15_26315) - 5702256..5702690 (-) 435 WP_015649419.1 hypothetical protein -
  R6I15_RS26320 (R6I15_26320) - 5702703..5703599 (-) 897 WP_003127513.1 Mu-like prophage major head subunit gpT family protein -
  R6I15_RS26325 (R6I15_26325) - 5703614..5704018 (-) 405 WP_003121593.1 hypothetical protein -
  R6I15_RS26330 (R6I15_26330) - 5704024..5705133 (-) 1110 WP_003129234.1 phage protease -
  R6I15_RS26335 (R6I15_26335) - 5705344..5705919 (-) 576 WP_010791815.1 phage virion morphogenesis protein -
  R6I15_RS26340 (R6I15_26340) - 5705921..5707159 (-) 1239 WP_003138159.1 PBECR2 nuclease fold domain-containing protein -
  R6I15_RS26345 (R6I15_26345) - 5707149..5708714 (-) 1566 WP_003126994.1 DUF935 domain-containing protein -
  R6I15_RS26350 (R6I15_26350) - 5708717..5710390 (-) 1674 WP_003126996.1 hypothetical protein -
  R6I15_RS26355 (R6I15_26355) - 5710392..5710940 (-) 549 WP_003121466.1 phage protein Gp27 family protein -
  R6I15_RS26360 (R6I15_26360) - 5710943..5711245 (-) 303 WP_003117314.1 hypothetical protein -
  R6I15_RS26365 (R6I15_26365) - 5711242..5711562 (-) 321 WP_003117315.1 DUF2730 family protein -
  R6I15_RS26370 (R6I15_26370) - 5711562..5712185 (-) 624 WP_019485965.1 Rz lysis protein -
  R6I15_RS26375 (R6I15_26375) - 5712387..5713016 (-) 630 WP_019485964.1 transglycosylase SLT domain-containing protein -
  R6I15_RS26380 (R6I15_26380) - 5713013..5713171 (-) 159 WP_019485963.1 hypothetical protein -
  R6I15_RS26385 (R6I15_26385) - 5713174..5713470 (-) 297 WP_003152187.1 putative holin -
  R6I15_RS26390 (R6I15_26390) - 5713585..5714082 (-) 498 WP_175587325.1 hypothetical protein -
  R6I15_RS26395 (R6I15_26395) - 5714137..5715210 (-) 1074 WP_023093882.1 hypothetical protein -
  R6I15_RS26400 (R6I15_26400) - 5715167..5715667 (-) 501 WP_019485961.1 hypothetical protein -
  R6I15_RS26405 (R6I15_26405) - 5715741..5716337 (-) 597 WP_019485960.1 hypothetical protein -
  R6I15_RS29275 - 5716343..5716756 (-) 414 WP_025991852.1 helix-turn-helix domain-containing protein -
  R6I15_RS26410 (R6I15_26410) - 5716842..5717051 (+) 210 WP_015649411.1 DNA-binding protein -
  R6I15_RS26415 (R6I15_26415) - 5717334..5717558 (-) 225 WP_015649410.1 hypothetical protein -
  R6I15_RS26420 (R6I15_26420) - 5717682..5718167 (+) 486 WP_003127832.1 hypothetical protein -
  R6I15_RS26425 (R6I15_26425) - 5718160..5718468 (+) 309 WP_019485959.1 helix-turn-helix domain-containing protein -
  R6I15_RS26430 (R6I15_26430) - 5718479..5719405 (+) 927 WP_019485958.1 DUF3102 domain-containing protein -
  R6I15_RS26435 (R6I15_26435) - 5719408..5721189 (+) 1782 WP_019485957.1 integrase catalytic domain-containing protein -
  R6I15_RS26440 (R6I15_26440) - 5721189..5722361 (+) 1173 WP_015649406.1 ExeA family protein -
  R6I15_RS26445 (R6I15_26445) - 5722363..5722704 (+) 342 WP_019485956.1 hypothetical protein -
  R6I15_RS26450 (R6I15_26450) - 5722701..5723144 (+) 444 WP_019485955.1 hypothetical protein -
  R6I15_RS26455 (R6I15_26455) - 5723144..5723758 (+) 615 WP_019485954.1 hypothetical protein -
  R6I15_RS26460 (R6I15_26460) - 5723751..5723951 (+) 201 WP_019485953.1 hypothetical protein -
  R6I15_RS26465 (R6I15_26465) - 5723944..5724567 (+) 624 WP_015649401.1 DUF3164 family protein -
  R6I15_RS26470 (R6I15_26470) - 5724569..5724865 (+) 297 WP_019485952.1 hypothetical protein -
  R6I15_RS26475 (R6I15_26475) - 5724852..5725418 (+) 567 WP_019485951.1 regulatory protein GemA -
  R6I15_RS26480 (R6I15_26480) - 5725624..5725992 (+) 369 WP_019485949.1 Mor transcription activator family protein -
  R6I15_RS26485 (R6I15_26485) pilP 5726097..5726543 (-) 447 Protein_5229 type 4a pilus biogenesis lipoprotein PilP -
  R6I15_RS26490 (R6I15_26490) pilO 5726540..5727163 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  R6I15_RS26495 (R6I15_26495) pilN 5727160..5727756 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  R6I15_RS26500 (R6I15_26500) pilM 5727756..5728820 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  R6I15_RS26505 (R6I15_26505) - 5729005..5731473 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37983.48 Da        Isoelectric Point: 4.5405

>NTDB_id=900285 R6I15_RS26500 WP_003110888.1 5727756..5728820(-) (pilM) [Pseudomonas aeruginosa isolate FELIX_MS463]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=900285 R6I15_RS26500 WP_003110888.1 5727756..5728820(-) (pilM) [Pseudomonas aeruginosa isolate FELIX_MS463]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.899

100

0.562
  comM Acinetobacter nosocomialis M2

55.899

100

0.562
  comM Acinetobacter baylyi ADP1

51.977

100

0.52
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466