Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   R6U80_RS05675 Genome accession   NZ_CP137623
Coordinates   1143120..1144442 (-) Length   440 a.a.
NCBI ID   WP_023189341.1    Uniprot ID   A0A0V8BZU5
Organism   Lactococcus lactis strain NCK401     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138120..1149442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6U80_RS05645 (R6U80_05645) - 1139271..1140239 (+) 969 WP_081041168.1 PhoH family protein -
  R6U80_RS05650 (R6U80_05650) - 1140297..1140776 (+) 480 WP_003130650.1 NUDIX hydrolase -
  R6U80_RS05655 (R6U80_05655) ybeY 1140928..1141416 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  R6U80_RS05660 (R6U80_05660) - 1141400..1141855 (+) 456 WP_003130653.1 diacylglycerol kinase family protein -
  R6U80_RS05665 (R6U80_05665) - 1141869..1142447 (+) 579 WP_313792064.1 nucleotidyltransferase family protein -
  R6U80_RS05670 (R6U80_05670) comFC 1142527..1142877 (-) 351 WP_313792063.1 hypothetical protein Machinery gene
  R6U80_RS05675 (R6U80_05675) comFA 1143120..1144442 (-) 1323 WP_023189341.1 DEAD/DEAH box helicase Machinery gene
  R6U80_RS05680 (R6U80_05680) - 1144499..1145128 (+) 630 WP_201247956.1 YigZ family protein -
  R6U80_RS05685 (R6U80_05685) - 1145262..1145759 (+) 498 WP_023189340.1 VanZ family protein -
  R6U80_RS05690 (R6U80_05690) - 1145807..1146826 (+) 1020 WP_313792062.1 DUF475 domain-containing protein -
  R6U80_RS05695 (R6U80_05695) - 1146941..1147687 (+) 747 WP_058223057.1 bifunctional metallophosphatase/5'-nucleotidase -
  R6U80_RS05700 (R6U80_05700) - 1147979..1148626 (+) 648 WP_058223056.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50249.76 Da        Isoelectric Point: 10.1065

>NTDB_id=899395 R6U80_RS05675 WP_023189341.1 1143120..1144442(-) (comFA) [Lactococcus lactis strain NCK401]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDNYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKITSVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=899395 R6U80_RS05675 WP_023189341.1 1143120..1144442(-) (comFA) [Lactococcus lactis strain NCK401]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAACTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTACTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(300-403)

Type III restriction enzyme, res subunit (ResIII)

(114-233)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0V8BZU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

37.029

100

0.38