Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R5R33_RS08465 Genome accession   NZ_CP137555
Coordinates   1962018..1963094 (-) Length   358 a.a.
NCBI ID   WP_318955583.1    Uniprot ID   A0AAU0N2H1
Organism   Microbulbifer pacificus strain SPO729     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1957018..1968094
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R33_RS08440 (R5R33_08440) aroK 1957537..1958058 (-) 522 WP_318955712.1 shikimate kinase AroK -
  R5R33_RS08445 (R5R33_08445) - 1958058..1960283 (-) 2226 WP_318955579.1 type IV pilus secretin PilQ family protein -
  R5R33_RS08450 (R5R33_08450) - 1960286..1960813 (-) 528 WP_318955580.1 pilus assembly protein PilP -
  R5R33_RS08455 (R5R33_08455) - 1960810..1961430 (-) 621 WP_318955581.1 type 4a pilus biogenesis protein PilO -
  R5R33_RS08460 (R5R33_08460) - 1961433..1962014 (-) 582 WP_318955582.1 PilN domain-containing protein -
  R5R33_RS08465 (R5R33_08465) pilM 1962018..1963094 (-) 1077 WP_318955583.1 pilus assembly protein PilM Machinery gene
  R5R33_RS08470 (R5R33_08470) - 1963250..1965940 (+) 2691 WP_318955584.1 penicillin-binding protein 1A -
  R5R33_RS08475 (R5R33_08475) - 1966039..1966524 (-) 486 WP_318955585.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39033.38 Da        Isoelectric Point: 4.3942

>NTDB_id=899039 R5R33_RS08465 WP_318955583.1 1962018..1963094(-) (pilM) [Microbulbifer pacificus strain SPO729]
MGMLPFLNKGPKAMLGLDISSTSVKLLELSRSGDKYRVESYATEPLPPNAVVDKNINDVGAIAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLEMPADLSDDALEAQISLEADQYIPYPLDEVNLDFEVQGPSERGEGQVEVLLAACRSENVEQRVSAL
TQAELQPGVVDVEAYAIERAFTLMENQFPAQEQLVVAVVDIGATMSALSVMVDGRIVYTREQLFGGRQLTDEIQRRYGLS
AEEATLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMDGLAEMVGQKLNKPTLV
ANPFRDMSVASRVNRQSLAVEAPSLMIAAGLAMREREF

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=899039 R5R33_RS08465 WP_318955583.1 1962018..1963094(-) (pilM) [Microbulbifer pacificus strain SPO729]
ATGGGGATGCTCCCTTTTCTCAACAAGGGCCCTAAAGCCATGCTTGGGCTCGATATTAGCTCCACCTCGGTAAAGTTGCT
TGAACTGAGTCGCAGTGGCGACAAATACCGTGTGGAAAGCTATGCAACCGAACCTCTGCCGCCAAACGCGGTAGTGGATA
AGAATATCAATGACGTGGGTGCCATTGCGGAGGCCATCCGCAAGGTAGTGCGGCGCTCCAAGACCCGTTTGCGCCAGGCT
GCGGTAGCAGTTTCCGGTTCCGCGGTCATCACCAAGACGCTGGAAATGCCGGCGGATCTCTCGGATGACGCGCTGGAGGC
GCAGATTTCGCTGGAAGCCGATCAATACATTCCCTACCCGCTGGATGAGGTGAACCTGGACTTTGAAGTGCAGGGGCCGT
CGGAGCGTGGCGAGGGGCAGGTCGAAGTGTTACTGGCGGCCTGCCGCAGCGAAAACGTGGAGCAGCGAGTTTCAGCGCTG
ACCCAGGCGGAGCTGCAGCCGGGGGTGGTTGATGTGGAGGCCTACGCCATTGAGCGGGCCTTCACCCTGATGGAAAACCA
GTTCCCCGCCCAGGAGCAACTGGTGGTGGCGGTTGTGGATATCGGTGCCACCATGAGTGCGCTGTCGGTGATGGTTGACG
GTCGCATTGTGTATACGCGCGAGCAGCTGTTTGGTGGGCGCCAGCTCACCGACGAGATCCAGCGCCGCTACGGCCTGTCT
GCCGAAGAGGCCACGCTGGCCAAGCGCCAGGGCGGCAGCGGCCTGCCGGACGACTACTACCCGGAAGTGCTGGAGCCCTT
CCGCGACGCGGTGGTTCAGCAAGTTACCCGCTCCCTGCAGTTCTTCTATTCCTCCACCTCCTACAGCGATGTCGATTACA
TCCTCCTCGGCGGTGGTGTAGCGGCAATGGACGGTCTCGCTGAAATGGTTGGTCAGAAACTGAATAAACCCACCCTGGTG
GCGAACCCCTTCCGTGACATGAGTGTGGCGTCCCGGGTCAACCGCCAGTCACTGGCGGTAGAGGCACCGTCGCTGATGAT
TGCCGCCGGCCTCGCCATGCGCGAGCGGGAGTTCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.841

98.324

0.52
  comM Acinetobacter nosocomialis M2

52.841

98.324

0.52
  comM Acinetobacter baylyi ADP1

48.295

98.324

0.475
  pilM Legionella pneumophila strain ERS1305867

44.382

99.441

0.441