Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R5R33_RS04315 Genome accession   NZ_CP137555
Coordinates   946136..947323 (-) Length   395 a.a.
NCBI ID   WP_318954816.1    Uniprot ID   A0AAU0N2L4
Organism   Microbulbifer pacificus strain SPO729     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 941136..952323
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5R33_RS04280 (R5R33_04280) - 941548..942462 (-) 915 WP_318954809.1 LysR family transcriptional regulator -
  R5R33_RS04285 (R5R33_04285) - 942610..943101 (+) 492 WP_318954810.1 DoxX family protein -
  R5R33_RS04290 (R5R33_04290) - 943165..944112 (+) 948 WP_318954811.1 glutathione S-transferase C-terminal domain-containing protein -
  R5R33_RS04295 (R5R33_04295) - 944187..944753 (+) 567 WP_318954812.1 heme-binding protein -
  R5R33_RS04300 (R5R33_04300) - 944776..945045 (-) 270 WP_318954813.1 high-potential iron-sulfur protein -
  R5R33_RS04305 (R5R33_04305) - 945160..945348 (-) 189 WP_318954814.1 hypothetical protein -
  R5R33_RS04310 (R5R33_04310) - 945540..946103 (+) 564 WP_318954815.1 NlpC/P60 family protein -
  R5R33_RS04315 (R5R33_04315) pilU 946136..947323 (-) 1188 WP_318954816.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R5R33_RS04320 (R5R33_04320) - 947508..948428 (+) 921 WP_318954817.1 polysaccharide deacetylase family protein -
  R5R33_RS04325 (R5R33_04325) - 948482..949306 (-) 825 WP_318954818.1 alpha/beta hydrolase -
  R5R33_RS04330 (R5R33_04330) - 949492..949878 (+) 387 WP_318954819.1 DUF2750 domain-containing protein -
  R5R33_RS04335 (R5R33_04335) - 949932..950897 (-) 966 WP_318954820.1 DUF4862 family protein -
  R5R33_RS04340 (R5R33_04340) - 951015..951878 (-) 864 WP_318954821.1 crotonase/enoyl-CoA hydratase family protein -

Sequence

Protein


Download         Length: 395 a.a.        Molecular weight: 44089.47 Da        Isoelectric Point: 6.0775

>NTDB_id=899030 R5R33_RS04315 WP_318954816.1 946136..947323(-) (pilU) [Microbulbifer pacificus strain SPO729]
MDFTGYLRVLAKNDGSDLYLSTGAPPCAKFQGVLKPLGKEPLAPGEIEKIAMEIMDEAQQEEFRRDLEMNLAMTIPNVGR
FRINIFWQRNQVSIVARNIVTEIPKFDDLKLPPILKDVVMAKRGLVLFVGGTGSGKSTSLAALIDHRNTNSGGHIITIED
PVEYVHRHKKSVINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLAISTLHANNANQALDRI
VNFFPEERRPQLLMSLSQNIRAFVSQRLVPTVEGKRCAAVEILLGTQTINELILKGEFHSIKEIMERSENLGMQTFDGAL
FKLYVEGRISLDDAIRNADSANNLRLRIKLYEEGKKPKAPASEAEGERSGVLKTATGFELSLESLDAEEEEAFQY

Nucleotide


Download         Length: 1188 bp        

>NTDB_id=899030 R5R33_RS04315 WP_318954816.1 946136..947323(-) (pilU) [Microbulbifer pacificus strain SPO729]
ATGGATTTTACCGGCTACCTTCGCGTACTTGCCAAAAATGATGGCTCAGACCTTTATCTCAGCACCGGTGCGCCGCCATG
CGCGAAATTCCAGGGCGTACTGAAGCCCCTGGGTAAAGAGCCGCTGGCTCCCGGGGAGATCGAGAAGATCGCCATGGAAA
TCATGGATGAGGCCCAGCAGGAGGAGTTCCGGCGCGATCTGGAAATGAACCTGGCCATGACCATCCCCAATGTGGGGCGT
TTCCGGATCAACATCTTCTGGCAGCGCAACCAGGTCTCGATTGTTGCGCGCAACATTGTCACCGAGATCCCGAAATTTGA
CGATCTGAAGTTGCCGCCGATTCTCAAAGACGTGGTGATGGCGAAGCGCGGACTGGTGCTGTTTGTCGGCGGTACCGGCT
CTGGTAAATCCACCTCGCTGGCGGCACTGATCGACCACCGCAATACCAACAGCGGTGGTCACATCATTACCATCGAAGAC
CCGGTCGAATATGTGCACCGGCACAAGAAGAGTGTGATCAACCAGCGTGAGGTAGGAGTCGATACCCGCAGCTTCCACGC
CGCACTGAAGAACACCCTGCGTCAGGCACCGGATGTCATTCTGATCGGCGAAATTCGCGACCGCGAAACCATGGAGCACG
CGATCGCGTTTGCGGAAACCGGCCATCTGGCGATTTCCACCCTGCATGCCAACAACGCCAACCAGGCATTGGACCGGATT
GTGAACTTCTTTCCGGAAGAGCGCCGCCCGCAGCTGCTGATGTCGCTGTCGCAGAATATCCGTGCGTTTGTTTCCCAGCG
TCTGGTCCCCACGGTCGAAGGCAAGCGCTGCGCGGCAGTGGAAATTCTGCTGGGTACCCAGACCATCAACGAGCTGATTC
TCAAGGGCGAGTTTCACTCCATCAAGGAGATCATGGAGCGCTCGGAAAACCTCGGCATGCAGACGTTTGACGGGGCGCTG
TTCAAACTCTATGTGGAAGGCCGTATCAGCCTCGATGACGCGATCCGCAATGCGGACTCCGCCAACAACCTGCGTCTGCG
TATCAAACTCTATGAGGAGGGCAAGAAGCCGAAGGCGCCGGCCTCGGAAGCCGAGGGCGAACGCAGTGGTGTGCTGAAAA
CCGCAACCGGTTTTGAGTTGTCCCTGGAGTCGCTGGATGCGGAAGAAGAGGAAGCCTTCCAGTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(105-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.152

100

0.522
  pilU Acinetobacter baylyi ADP1

53.482

90.886

0.486
  pilU Vibrio cholerae strain A1552

50

88.608

0.443
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

85.823

0.372