Detailed information NTG0898627

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5016_RS20985
• Genome accession: NZ_CP137507
• Coordinates: 4528848..4529996 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA0698
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4523848..4534996

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5016_RS20965 (R5016_20965)	-	4524585..4525769 (-)	1185	WP_003084564.1	cystathionine gamma-synthase	-
R5016_RS20970 (R5016_20970)	-	4525766..4527139 (-)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
R5016_RS20975 (R5016_20975)	-	4527382..4527786 (-)	405	WP_003084560.1	NINE protein	-
R5016_RS20980 (R5016_20980)	czcD	4527941..4528840 (+)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
R5016_RS20985 (R5016_20985)	pilU	4528848..4529996 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5016_RS20990 (R5016_20990)	pilT	4530174..4531208 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5016_RS20995 (R5016_20995)	-	4531423..4532115 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5016_RS21000 (R5016_21000)	proC	4532127..4532948 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5016_RS21005 (R5016_21005)	-	4532959..4533552 (+)	594	WP_003084544.1	YggT family protein	-
R5016_RS21010 (R5016_21010)	-	4533549..4533845 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898627 R5016_RS20985 WP_003100959.1 4528848..4529996(-) (pilU) [Pseudomonas aeruginosa strain HPA0698]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898627 R5016_RS20985 WP_003100959.1 4528848..4529996(-) (pilU) [Pseudomonas aeruginosa strain HPA0698]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382