Detailed information NTG0898493

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5017_RS25335
• Genome accession: NZ_CP137498
• Coordinates: 5356588..5357736 (-)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain HPA0663
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5351588..5362736

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5017_RS25315 (R5017_25315)	-	5352325..5353509 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
R5017_RS25320 (R5017_25320)	-	5353506..5354879 (-)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
R5017_RS25325 (R5017_25325)	-	5355122..5355526 (-)	405	WP_003084560.1	NINE protein	-
R5017_RS25330 (R5017_25330)	czcD	5355681..5356580 (+)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
R5017_RS25335 (R5017_25335)	pilU	5356588..5357736 (-)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
R5017_RS25340 (R5017_25340)	pilT	5357914..5358948 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5017_RS25345 (R5017_25345)	-	5359163..5359855 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5017_RS25350 (R5017_25350)	proC	5359867..5360688 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5017_RS25355 (R5017_25355)	-	5360699..5361292 (+)	594	WP_003084544.1	YggT family protein	-
R5017_RS25360 (R5017_25360)	-	5361289..5361585 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=898493 R5017_RS25335 WP_003110016.1 5356588..5357736(-) (pilU) [Pseudomonas aeruginosa strain HPA0663]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898493 R5017_RS25335 WP_003110016.1 5356588..5357736(-) (pilU) [Pseudomonas aeruginosa strain HPA0663]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382