Detailed information NTG0898446

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5026_RS08505
• Genome accession: NZ_CP137495
• Coordinates: 1790113..1791261 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA0875
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1785113..1796261

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5026_RS08480 (R5026_08480)	-	1786264..1786560 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5026_RS08485 (R5026_08485)	-	1786557..1787150 (-)	594	WP_003084544.1	YggT family protein	-
R5026_RS08490 (R5026_08490)	proC	1787161..1787982 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5026_RS08495 (R5026_08495)	-	1787994..1788686 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5026_RS08500 (R5026_08500)	pilT	1788901..1789935 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5026_RS08505 (R5026_08505)	pilU	1790113..1791261 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5026_RS08510 (R5026_08510)	czcD	1791269..1792168 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
R5026_RS08515 (R5026_08515)	-	1792323..1792727 (+)	405	WP_003084560.1	NINE protein	-
R5026_RS08520 (R5026_08520)	-	1792970..1794343 (+)	1374	WP_126892857.1	pyridoxal-phosphate dependent enzyme	-
R5026_RS08525 (R5026_08525)	-	1794340..1795524 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898446 R5026_RS08505 WP_003100959.1 1790113..1791261(+) (pilU) [Pseudomonas aeruginosa strain HPA0875]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898446 R5026_RS08505 WP_003100959.1 1790113..1791261(+) (pilU) [Pseudomonas aeruginosa strain HPA0875]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382