Detailed information NTG0898416

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5030_RS11785
• Genome accession: NZ_CP137493
• Coordinates: 2505072..2506220 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain HPA1346
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2500072..2511220

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5030_RS11760 (R5030_11760)	-	2501223..2501519 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5030_RS11765 (R5030_11765)	-	2501516..2502109 (-)	594	WP_003084544.1	YggT family protein	-
R5030_RS11770 (R5030_11770)	proC	2502120..2502941 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5030_RS11775 (R5030_11775)	-	2502953..2503645 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5030_RS11780 (R5030_11780)	pilT	2503860..2504894 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5030_RS11785 (R5030_11785)	pilU	2505072..2506220 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
R5030_RS11790 (R5030_11790)	czcD	2506228..2507127 (-)	900	WP_023913256.1	CDF family zinc transporter CzcD	-
R5030_RS11795 (R5030_11795)	-	2507282..2507686 (+)	405	WP_003084560.1	NINE protein	-
R5030_RS11800 (R5030_11800)	-	2507929..2509302 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
R5030_RS11805 (R5030_11805)	-	2509299..2510483 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=898416 R5030_RS11785 WP_003110016.1 2505072..2506220(+) (pilU) [Pseudomonas aeruginosa strain HPA1346]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898416 R5030_RS11785 WP_003110016.1 2505072..2506220(+) (pilU) [Pseudomonas aeruginosa strain HPA1346]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382