Detailed information NTG0898343

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R5019_RS07890
• Genome accession: NZ_CP137490
• Coordinates: 1694837..1695985 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HPA2017
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1689837..1700985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R5019_RS07865 (R5019_07865)	-	1690988..1691284 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
R5019_RS07870 (R5019_07870)	-	1691281..1691874 (-)	594	WP_003084544.1	YggT family protein	-
R5019_RS07875 (R5019_07875)	proC	1691885..1692706 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
R5019_RS07880 (R5019_07880)	-	1692718..1693410 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
R5019_RS07885 (R5019_07885)	pilT	1693625..1694659 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
R5019_RS07890 (R5019_07890)	pilU	1694837..1695985 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
R5019_RS07895 (R5019_07895)	czcD	1695993..1696892 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
R5019_RS07900 (R5019_07900)	-	1697046..1697450 (+)	405	WP_003084560.1	NINE protein	-
R5019_RS07905 (R5019_07905)	-	1697693..1699066 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
R5019_RS07910 (R5019_07910)	-	1699063..1700247 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=898343 R5019_RS07890 WP_003100959.1 1694837..1695985(+) (pilU) [Pseudomonas aeruginosa strain HPA2017]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=898343 R5019_RS07890 WP_003100959.1 1694837..1695985(+) (pilU) [Pseudomonas aeruginosa strain HPA2017]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382