Detailed information NTG0896720

Bioinformatically predicted

Overview

• Name: ssb
• Type: Machinery gene
• Locus tag: R4T13_RS12240
• Genome accession: NZ_CP137179
• Coordinates: 2512908..2513432 (+)
• Length: 174 a.a.
• NCBI ID: WP_003826621.1
• Uniprot ID: A0A5B0T4S2
• Organism: Citrobacter freundii strain F4321
• Function: ssDNA binding (predicted from homology) DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2513597..2527299	2512908..2513432	flank	165

Gene organization within MGE regions

Location: 2512908..2527299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4T13_RS12240 (R4T13_12240)	ssb	2512908..2513432 (+)	525	WP_003826621.1	single-stranded DNA-binding protein SSB1	Machinery gene
R4T13_RS12245 (R4T13_12245)	-	2513597..2514922 (+)	1326	WP_204787547.1	site-specific integrase	-
R4T13_RS12250 (R4T13_12250)	-	2514915..2516483 (+)	1569	WP_193557062.1	site-specific integrase	-
R4T13_RS12255 (R4T13_12255)	-	2516493..2518370 (+)	1878	WP_139393187.1	integrase	-
R4T13_RS12260 (R4T13_12260)	-	2518358..2518837 (+)	480	WP_139388012.1	hypothetical protein	-
R4T13_RS12265 (R4T13_12265)	-	2518834..2519274 (+)	441	WP_139388013.1	VPA1267 family protein	-
R4T13_RS12270 (R4T13_12270)	-	2519271..2521433 (+)	2163	WP_139388014.1	AAA family ATPase	-
R4T13_RS12275 (R4T13_12275)	-	2521651..2521812 (+)	162	Protein_2413	IS5/IS1182 family transposase	-
R4T13_RS12280 (R4T13_12280)	-	2521971..2522639 (+)	669	WP_017780890.1	hypothetical protein	-
R4T13_RS12285 (R4T13_12285)	tet(E)	2522727..2523944 (-)	1218	WP_017780889.1	tetracycline efflux MFS transporter Tet(E)	-
R4T13_RS12290 (R4T13_12290)	tetR	2524025..2524660 (+)	636	WP_017780888.1	tetracycline resistance transcriptional repressor TetR	-
R4T13_RS12295 (R4T13_12295)	-	2524992..2525183 (-)	192	WP_017780887.1	hypothetical protein	-
R4T13_RS12300 (R4T13_12300)	-	2525393..2525863 (-)	471	WP_017780886.1	hypothetical protein	-
R4T13_RS12305 (R4T13_12305)	-	2525962..2526270 (+)	309	WP_047440196.1	helix-turn-helix domain-containing protein	-
R4T13_RS12310 (R4T13_12310)	-	2526379..2527299 (-)	921	WP_017780885.1	DUF4113 domain-containing protein	-

Sequence

Protein

• Length: 174 a.a.
• Molecular weight: 18621.66 Da
• Isoelectric Point: 5.2456

>NTDB_id=896720 R4T13_RS12240 WP_003826621.1 2512908..2513432(+) (ssb) [Citrobacter freundii strain F4321]
MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYI
EGQLRTRKWTDQSGVEKYTTEVVVNVGGTMQMLGGRQGGGAPAGGGQQQGGWGQPQQPQGGNQFSGGAQSRPQQSAPAAP
SNEPPMDFDDDIPF

Nucleotide

• Length: 525 bp

>NTDB_id=896720 R4T13_RS12240 WP_003826621.1 2512908..2513432(+) (ssb) [Citrobacter freundii strain F4321]
ATGGCCAGCAGAGGCGTAAACAAGGTGATTCTCGTCGGTAATCTGGGCCAGGACCCGGAAGTACGCTATATGCCGAATGG
TGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGTGAAATGAAAGAGCAGACTG
AATGGCACCGCGTTGTGCTGTTTGGCAAACTGGCGGAAGTGGCCAGTGAATATCTGCGTAAAGGTTCCCAGGTCTATATC
GAAGGTCAGCTGCGTACCCGCAAATGGACCGATCAGTCCGGCGTAGAAAAGTACACCACTGAGGTTGTTGTTAACGTTGG
CGGCACCATGCAAATGCTGGGCGGTCGTCAGGGCGGTGGTGCTCCGGCAGGTGGCGGCCAGCAGCAGGGTGGTTGGGGTC
AGCCTCAGCAGCCGCAGGGCGGCAATCAGTTCAGCGGCGGCGCGCAGTCTCGTCCGCAGCAGTCCGCTCCGGCAGCGCCG
TCTAACGAACCGCCGATGGATTTCGACGACGATATTCCGTTCTAG

Domains

Predicted by InterproScan.

• Single-strand binding protein family (SSB) (6-112)

3D structure

• AlphaFold DB: A0A5B0T4S2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ssb	Vibrio cholerae strain A1552	73.889	100	0.764
ssb	Glaesserella parasuis strain SC1401	56.831	100	0.598
ssb	Neisseria meningitidis MC58	48.603	100	0.5
ssb	Neisseria gonorrhoeae MS11	48.603	100	0.5