NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	AAVB50_RS09755	Genome accession	NZ_CP154923
Coordinates	1801715..1802332 (+)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	P39694
Organism	Bacillus subtilis strain FUA2233
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1796715..1807332

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAVB50_RS09720 (AAVB50_09715)	aroE	1797379..1798221 (+)	843	WP_038429328.1	shikimate dehydrogenase	-
AAVB50_RS09725 (AAVB50_09720)	yhbY	1798215..1798505 (+)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
AAVB50_RS09730 (AAVB50_09725)	nadD	1798517..1799086 (+)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
AAVB50_RS09735 (AAVB50_09730)	yqeK	1799076..1799636 (+)	561	WP_029317884.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AAVB50_RS09740 (AAVB50_09735)	rsfS	1799654..1800004 (+)	351	WP_345806061.1	ribosome silencing factor	-
AAVB50_RS09745 (AAVB50_09740)	yqeM	1800001..1800744 (+)	744	WP_106073582.1	class I SAM-dependent methyltransferase	-
AAVB50_RS09750 (AAVB50_09745)	comER	1800810..1801631 (-)	822	WP_004398597.1	late competence protein ComER	-
AAVB50_RS09755 (AAVB50_09750)	comEA	1801715..1802332 (+)	618	WP_004398514.1	competence protein ComEA	Machinery gene
AAVB50_RS09760 (AAVB50_09755)	comEB	1802399..1802968 (+)	570	WP_015714288.1	ComE operon protein 2	-
AAVB50_RS09765 (AAVB50_09760)	comEC	1802965..1805295 (+)	2331	WP_345806062.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAVB50_RS09770 (AAVB50_09765)	yqzM	1805335..1805469 (-)	135	WP_003229983.1	YqzM family protein	-
AAVB50_RS09775 (AAVB50_09770)	-	1805510..1805659 (+)	150	WP_003229985.1	hypothetical protein	-
AAVB50_RS09780 (AAVB50_09775)	holA	1805699..1806742 (+)	1044	WP_345806063.1	DNA polymerase III subunit delta	-
AAVB50_RS09785 (AAVB50_09780)	rpsT	1806757..1807023 (-)	267	WP_003229989.1	30S ribosomal protein S20	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=895256 AAVB50_RS09755 WP_004398514.1 1801715..1802332(+) (comEA) [Bacillus subtilis strain FUA2233]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=895256 AAVB50_RS09755 WP_004398514.1 1801715..1802332(+) (comEA) [Bacillus subtilis strain FUA2233]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
PDB	8DFK

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0895256