Detailed information NTG0894737

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R3L05_RS10375
• Genome accession: NZ_CP137071
• Coordinates: 2117239..2118357 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain SNUBHAB0220
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2112239..2123357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R3L05_RS10365 (R3L05_10375)	-	2115355..2116047 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
R3L05_RS10370 (R3L05_10380)	pilT	2116174..2117211 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
R3L05_RS10375 (R3L05_10385)	pilU	2117239..2118357 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R3L05_RS10380 (R3L05_10390)	fur	2118427..2118864 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
R3L05_RS10385 (R3L05_10395)	-	2118976..2119374 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
R3L05_RS10390 (R3L05_10400)	-	2119429..2119752 (-)	324	WP_000442587.1	RnfH family protein	-
R3L05_RS10395 (R3L05_10405)	-	2119752..2120819 (-)	1068	WP_002019572.1	hypothetical protein	-
R3L05_RS10400 (R3L05_10410)	-	2120972..2121427 (-)	456	WP_000782976.1	bacteriohemerythrin	-
R3L05_RS10405 (R3L05_10415)	-	2121680..2122528 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=894737 R3L05_RS10375 WP_000347039.1 2117239..2118357(+) (pilU) [Acinetobacter baumannii strain SNUBHAB0220]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=894737 R3L05_RS10375 WP_000347039.1 2117239..2118357(+) (pilU) [Acinetobacter baumannii strain SNUBHAB0220]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395