Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   R3Y73_RS03015 Genome accession   NZ_CP137015
Coordinates   656168..656782 (-) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain CYS06     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 651168..661782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3Y73_RS02985 (R3Y73_02985) rpsT 651468..651734 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  R3Y73_RS02990 (R3Y73_02990) holA 651751..652792 (-) 1042 Protein_593 DNA polymerase III subunit delta -
  R3Y73_RS02995 (R3Y73_02995) - 652832..652986 (-) 155 Protein_594 hypothetical protein -
  R3Y73_RS03000 (R3Y73_03000) - 653027..653161 (+) 135 WP_003152870.1 YqzM family protein -
  R3Y73_RS03005 (R3Y73_03005) comEC 653180..655531 (-) 2352 WP_077722611.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  R3Y73_RS03010 (R3Y73_03010) - 655532..656101 (-) 570 WP_003152868.1 ComE operon protein 2 -
  R3Y73_RS03015 (R3Y73_03015) comEA 656168..656782 (-) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  R3Y73_RS03020 (R3Y73_03020) comER 656841..657662 (+) 822 WP_014305449.1 late competence protein ComER -
  R3Y73_RS03025 (R3Y73_03025) - 657731..658468 (-) 738 WP_031378935.1 class I SAM-dependent methyltransferase -
  R3Y73_RS03030 (R3Y73_03030) rsfS 658465..658821 (-) 357 WP_003152864.1 ribosome silencing factor -
  R3Y73_RS03035 (R3Y73_03035) yqeK 658839..659399 (-) 561 WP_077722612.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  R3Y73_RS03040 (R3Y73_03040) - 659389..659958 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  R3Y73_RS03045 (R3Y73_03045) yhbY 659969..660259 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  R3Y73_RS03050 (R3Y73_03050) aroE 660253..661095 (-) 843 WP_015417858.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=894323 R3Y73_RS03015 WP_013352942.1 656168..656782(-) (comEA) [Bacillus velezensis strain CYS06]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=894323 R3Y73_RS03015 WP_013352942.1 656168..656782(-) (comEA) [Bacillus velezensis strain CYS06]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363