Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   AABJ97_RS10450 Genome accession   NZ_AP027709
Coordinates   2116100..2117110 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain PSS-15     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2111100..2122110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AABJ97_RS10415 (VEE23_20330) nudB 2112460..2112912 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  AABJ97_RS10420 (VEE23_20340) yebC 2112941..2113681 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  AABJ97_RS10425 (VEE23_20350) ruvC 2113716..2114237 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  AABJ97_RS10430 - 2114239..2114418 (-) 180 Protein_2036 hypothetical protein -
  AABJ97_RS10440 yobI 2115276..2115341 (+) 66 WP_001386853.1 stress response small protein YobI -
  AABJ97_RS10445 (VEE23_20380) ruvA 2115480..2116091 (+) 612 WP_000580328.1 Holliday junction branch migration protein RuvA -
  AABJ97_RS10450 (VEE23_20390) ruvB 2116100..2117110 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  AABJ97_RS10455 (VEE23_20400) znuB 2117257..2118042 (-) 786 WP_000571465.1 zinc ABC transporter permease subunit ZnuB -
  AABJ97_RS10460 (VEE23_20410) znuC 2118039..2118794 (-) 756 WP_134232785.1 zinc ABC transporter ATP-binding protein ZnuC -
  AABJ97_RS10465 (VEE23_20420) znuA 2118873..2119805 (+) 933 WP_001342995.1 zinc ABC transporter substrate-binding protein ZnuA -
  AABJ97_RS10470 (VEE23_20430) mepM 2119821..2121143 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=89431 AABJ97_RS10450 WP_000568519.1 2116100..2117110(+) (ruvB) [Escherichia coli strain PSS-15]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=89431 AABJ97_RS10450 WP_000568519.1 2116100..2117110(+) (ruvB) [Escherichia coli strain PSS-15]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494