Detailed information NTG0893997

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R3J30_RS05000
• Genome accession: NZ_CP136980
• Coordinates: 1079351..1080481 (-)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain CFBP8417
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1074351..1085481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R3J30_RS04980 (R3J30_04970)	-	1074385..1075143 (+)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-
R3J30_RS04985 (R3J30_04975)	hemC	1075151..1076068 (+)	918	WP_004084955.1	hydroxymethylbilane synthase	-
R3J30_RS04990 (R3J30_04980)	-	1076122..1076931 (-)	810	WP_021358437.1	YdiY family protein	-
R3J30_RS04995 (R3J30_04985)	-	1077714..1078682 (+)	969	WP_004084952.1	YafY family protein	-
R3J30_RS05000 (R3J30_04990)	pilU	1079351..1080481 (-)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R3J30_RS05005 (R3J30_04995)	pilT	1080636..1081670 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
R3J30_RS05010 (R3J30_05000)	-	1082292..1082504 (+)	213	WP_021358439.1	hypothetical protein	-
R3J30_RS05015 (R3J30_05005)	-	1082889..1083716 (+)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
R3J30_RS05020 (R3J30_05010)	-	1084333..1085157 (-)	825	WP_004084943.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=893997 R3J30_RS05000 WP_004084950.1 1079351..1080481(-) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8417]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=893997 R3J30_RS05000 WP_004084950.1 1079351..1080481(-) (pilU) [Xylella fastidiosa subsp. multiplex strain CFBP8417]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402