Detailed information NTG0893881

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R3J26_RS06145
• Genome accession: NZ_CP136970
• Coordinates: 1352250..1353380 (+)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain XF3348
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1347250..1358380

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R3J26_RS06125 (R3J26_06110)	-	1347574..1348398 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
R3J26_RS06130 (R3J26_06115)	-	1349015..1349842 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
R3J26_RS06135 (R3J26_06120)	-	1350227..1350439 (-)	213	WP_021358439.1	hypothetical protein	-
R3J26_RS06140 (R3J26_06125)	pilT	1351061..1352095 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
R3J26_RS06145 (R3J26_06130)	pilU	1352250..1353380 (+)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R3J26_RS06150 (R3J26_06135)	-	1354049..1355017 (-)	969	WP_004084952.1	YafY family protein	-
R3J26_RS06155 (R3J26_06140)	-	1355800..1356609 (+)	810	WP_021358437.1	YdiY family protein	-
R3J26_RS06160 (R3J26_06145)	hemC	1356663..1357580 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
R3J26_RS06165 (R3J26_06150)	-	1357588..1358346 (-)	759	WP_004084957.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=893881 R3J26_RS06145 WP_004084950.1 1352250..1353380(+) (pilU) [Xylella fastidiosa subsp. multiplex strain XF3348]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=893881 R3J26_RS06145 WP_004084950.1 1352250..1353380(+) (pilU) [Xylella fastidiosa subsp. multiplex strain XF3348]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402