Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   R0135_RS12735 Genome accession   NZ_CP136864
Coordinates   2864469..2865533 (+) Length   354 a.a.
NCBI ID   WP_407347238.1    Uniprot ID   -
Organism   Congregibacter variabilis strain IMCC43200     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2859469..2870533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R0135_RS12730 (R0135_12690) - 2861820..2864252 (-) 2433 WP_407347237.1 penicillin-binding protein 1A -
  R0135_RS12735 (R0135_12695) pilM 2864469..2865533 (+) 1065 WP_407347238.1 pilus assembly protein PilM Machinery gene
  R0135_RS12740 (R0135_12700) - 2865533..2866099 (+) 567 WP_407347239.1 PilN domain-containing protein -
  R0135_RS12745 (R0135_12705) - 2866103..2866717 (+) 615 WP_407347240.1 type 4a pilus biogenesis protein PilO -
  R0135_RS12750 (R0135_12710) - 2866717..2867250 (+) 534 WP_407347241.1 pilus assembly protein PilP -
  R0135_RS12755 (R0135_12715) pilQ 2867300..2869441 (+) 2142 WP_407347242.1 type IV pilus secretin PilQ -
  R0135_RS12760 (R0135_12720) aroK 2869518..2869994 (+) 477 WP_407349920.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38565.05 Da        Isoelectric Point: 4.2362

>NTDB_id=893165 R0135_RS12735 WP_407347238.1 2864469..2865533(+) (pilM) [Congregibacter variabilis strain IMCC43200]
MLGLLGKRKQTPLLGIDISSTTVKLLELSRTGDKYRVESYAVCSLPQDAVVEKAINDVDGVSNAVRSVVAQSRSKLKLAS
TAVAGSSVITKLIDMPSGLSDDEMETQLTLEADQYIPYPLEEVALDFEVQGPSAERENMVEVLLAACRRETIDLRVEAIE
GADLEAKIVDVEAYAMERSYALIREHLELKEDSIVAIVDIGATMTTLSVLKNGNTIYTREQLFGGKQLTDEIMRRYGLPL
EEAGLAKKQGGLPDDYEPELLNPFRDAVVQQVTRSLQLFFSSSQYNDVDYIIMAGGVSFMDGLSELVQQQLGTPTVVANP
FAEMTISPRVNAVALGSDAPAMMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=893165 R0135_RS12735 WP_407347238.1 2864469..2865533(+) (pilM) [Congregibacter variabilis strain IMCC43200]
TTGCTTGGGTTGCTAGGAAAACGTAAGCAGACGCCGCTCTTGGGGATTGATATTAGCTCCACGACGGTCAAGCTGCTGGA
GCTCAGTCGCACTGGGGACAAGTATCGCGTAGAGAGTTACGCGGTGTGTTCGCTCCCCCAGGACGCTGTAGTTGAGAAAG
CCATCAATGATGTTGATGGTGTGTCTAACGCTGTGCGCAGCGTGGTGGCTCAATCACGCTCCAAACTTAAGCTGGCATCC
ACCGCGGTGGCCGGCTCTTCAGTCATTACCAAACTGATCGATATGCCTTCTGGCCTCTCCGATGATGAGATGGAGACGCA
GCTCACCCTGGAGGCAGACCAGTACATTCCCTATCCCTTGGAAGAGGTTGCGCTCGATTTTGAAGTGCAAGGCCCATCGG
CCGAGAGAGAAAACATGGTCGAGGTGCTGTTGGCAGCCTGTCGCAGAGAGACCATCGACCTTCGCGTCGAGGCCATCGAA
GGTGCTGACCTGGAGGCCAAAATAGTTGATGTTGAGGCCTACGCCATGGAGCGTTCTTACGCTCTCATCCGCGAGCATCT
CGAACTCAAAGAAGACAGTATCGTCGCCATCGTCGATATCGGCGCGACCATGACCACCCTGAGCGTTCTCAAAAACGGTA
ATACTATTTATACACGCGAGCAGCTTTTCGGTGGCAAGCAACTTACCGACGAGATCATGCGCCGTTACGGCTTGCCTCTT
GAGGAAGCCGGTCTTGCCAAGAAGCAGGGCGGACTTCCCGATGATTATGAGCCGGAGTTGCTCAATCCGTTTCGAGATGC
GGTGGTGCAGCAGGTCACGAGATCACTCCAGTTGTTTTTCTCTTCCAGTCAGTACAACGATGTCGATTACATCATCATGG
CTGGCGGTGTGTCATTTATGGACGGCCTGTCGGAGCTGGTTCAACAGCAGCTCGGCACGCCAACCGTGGTTGCAAACCCC
TTTGCTGAGATGACGATTTCTCCGAGAGTGAATGCCGTGGCTTTAGGCAGTGATGCTCCAGCCATGATGATTGCCTGCGG
TTTGGCGCTGCGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(15-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.571

98.87

0.52
  comM Acinetobacter nosocomialis M2

52.571

98.87

0.52
  comM Acinetobacter baylyi ADP1

49.143

98.87

0.486
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466