Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AAH969_RS30200 Genome accession   NZ_CP154349
Coordinates   6452988..6453611 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PAE3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6447988..6458611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAH969_RS30180 (AAH969_30190) aroB 6448583..6449689 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  AAH969_RS30185 (AAH969_30195) aroK 6449739..6450257 (-) 519 WP_003095833.1 shikimate kinase AroK -
  AAH969_RS30190 (AAH969_30200) pilQ 6450269..6452413 (-) 2145 WP_003123619.1 type 4a pilus secretin PilQ Machinery gene
  AAH969_RS30195 (AAH969_30205) comL/pilP 6452467..6452991 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  AAH969_RS30200 (AAH969_30210) pilO 6452988..6453611 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAH969_RS30205 (AAH969_30215) pilN 6453608..6454204 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  AAH969_RS30210 (AAH969_30220) pilM 6454204..6455268 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  AAH969_RS30215 (AAH969_30225) - 6455453..6457924 (+) 2472 WP_031756300.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=893141 AAH969_RS30200 WP_003103268.1 6452988..6453611(-) (pilO) [Pseudomonas aeruginosa strain PAE3]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=893141 AAH969_RS30200 WP_003103268.1 6452988..6453611(-) (pilO) [Pseudomonas aeruginosa strain PAE3]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401