Detailed information NTG0892751

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M0M55_RS01990
• Genome accession: NZ_CP136800
• Coordinates: 421792..422940 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain A6-17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 416792..427940

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M0M55_RS01965 (M0M55_01965)	-	417943..418239 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M0M55_RS01970 (M0M55_01970)	-	418236..418829 (-)	594	WP_003084544.1	YggT family protein	-
M0M55_RS01975 (M0M55_01975)	proC	418840..419661 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M0M55_RS01980 (M0M55_01980)	-	419673..420365 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M0M55_RS01985 (M0M55_01985)	pilT	420580..421614 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M0M55_RS01990 (M0M55_01990)	pilU	421792..422940 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
M0M55_RS01995 (M0M55_01995)	czcD	422948..423847 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M0M55_RS02000 (M0M55_02000)	-	424001..424405 (+)	405	WP_003084560.1	NINE protein	-
M0M55_RS02005 (M0M55_02005)	-	424648..426021 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
M0M55_RS02010 (M0M55_02010)	-	426018..427202 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=892751 M0M55_RS01990 WP_003110016.1 421792..422940(+) (pilU) [Pseudomonas aeruginosa strain A6-17]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=892751 M0M55_RS01990 WP_003110016.1 421792..422940(+) (pilU) [Pseudomonas aeruginosa strain A6-17]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382