Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R1T46_RS00365 Genome accession   NZ_CP136693
Coordinates   78730..79851 (-) Length   373 a.a.
NCBI ID   WP_041332635.1    Uniprot ID   W5YPF4
Organism   Marinobacter salarius strain CI.11.F.A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 73730..84851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R1T46_RS00350 (R1T46_00350) polA 73854..76589 (+) 2736 WP_317307021.1 DNA polymerase I -
  R1T46_RS00355 (R1T46_00355) - 76810..77178 (+) 369 WP_041332636.1 hypothetical protein -
  R1T46_RS00360 (R1T46_00360) - 77208..78614 (-) 1407 WP_317307022.1 OmpP1/FadL family transporter -
  R1T46_RS00365 (R1T46_00365) pilU 78730..79851 (-) 1122 WP_041332635.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R1T46_RS00370 (R1T46_00370) pilT 80026..81060 (-) 1035 WP_027830795.1 type IV pilus twitching motility protein PilT Machinery gene
  R1T46_RS00375 (R1T46_00375) - 81206..81898 (+) 693 WP_317307023.1 YggS family pyridoxal phosphate-dependent enzyme -
  R1T46_RS00380 (R1T46_00380) proC 81982..82815 (+) 834 WP_317307024.1 pyrroline-5-carboxylate reductase -
  R1T46_RS00385 (R1T46_00385) - 82839..83420 (+) 582 WP_036203140.1 YggT family protein -
  R1T46_RS21570 - 83664..84473 (+) 810 Protein_71 dynamin family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41497.65 Da        Isoelectric Point: 6.3471

>NTDB_id=892481 R1T46_RS00365 WP_041332635.1 78730..79851(-) (pilU) [Marinobacter salarius strain CI.11.F.A3]
MEFEKLLRLMVDKGGSDLFITAGVPPSMKVNGKVLPVTKNALTPEQTREFVYGAMNDKQRVEFEESHECNFAISARGIGR
FRVSAFFQRNLCGMVLRRIEVKIPQIDDLALPEIIKDLAMTKRGLIMFVGATGTGKSTSLAAMLGHRNRNSRGHIISIED
PIEFVHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHNQIWMDLSLNLKAIVAQQLIPTPDGQGRKAVIEVLINTPLVADLIRKGEVHRLKELMSKSNESGMRTFDQA
LYELYAEGSITYEDALAHADSANDLRLMIKLGADSQGADKLASSVDKLSIQDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=892481 R1T46_RS00365 WP_041332635.1 78730..79851(-) (pilU) [Marinobacter salarius strain CI.11.F.A3]
ATGGAATTCGAAAAACTGCTCCGTTTGATGGTGGACAAAGGCGGCTCCGACCTTTTCATTACTGCCGGTGTGCCACCCTC
CATGAAGGTCAACGGCAAGGTCCTGCCTGTCACCAAGAATGCATTGACGCCGGAGCAGACGAGGGAGTTCGTCTATGGCG
CCATGAACGACAAACAGCGGGTCGAATTCGAAGAAAGCCACGAGTGTAACTTCGCCATCAGTGCCCGAGGCATCGGTCGC
TTCCGTGTCAGCGCCTTCTTCCAGCGCAACCTCTGCGGCATGGTGCTGCGGCGGATCGAGGTGAAAATCCCGCAGATTGA
TGACCTGGCACTGCCGGAAATCATCAAGGATCTGGCCATGACCAAGCGTGGACTGATCATGTTCGTGGGTGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCTATGCTCGGGCATCGTAACCGCAACAGCCGGGGCCACATCATCTCCATCGAAGAC
CCCATCGAATTCGTGCACCAGCACCAGGGCTGTATCGTGACCCAGCGAGAGGTGGGCATCGATACCGATAGCTTTGAAGT
GGCTCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCCTCATCGGTGAGGTGCGTACCCGCCAGACCATGGAATACT
CTGTGCAGTTTGCTGAAACCGGCCACCTGTGCCTCGCTACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCCAGTTCTTCCCGCCGGAACAGCACAACCAGATCTGGATGGACCTGTCCCTGAACCTCAAGGCCATCGTCGCTCAACA
GTTGATCCCGACGCCGGACGGGCAGGGCCGCAAAGCCGTCATTGAAGTGTTGATCAACACACCGTTGGTCGCGGACCTGA
TTCGAAAAGGCGAAGTGCACCGACTTAAGGAACTGATGTCCAAGTCCAACGAGAGCGGCATGCGCACCTTTGATCAGGCG
CTTTATGAACTCTATGCCGAAGGCTCCATCACTTATGAGGATGCCCTCGCGCACGCGGATTCAGCCAACGACCTGCGCCT
GATGATCAAGCTGGGTGCCGATTCCCAGGGCGCTGACAAGCTGGCTTCCAGTGTCGACAAGTTGTCGATCCAGGACGATT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W5YPF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

78.437

99.464

0.78
  pilU Acinetobacter baylyi ADP1

63.243

99.196

0.627
  pilU Vibrio cholerae strain A1552

57.429

93.834

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

90.885

0.394
  pilT Acinetobacter baumannii strain A118

41.194

89.812

0.37
  pilT Acinetobacter baumannii D1279779

41.194

89.812

0.37
  pilT Acinetobacter nosocomialis M2

40.896

89.812

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.812

0.365
  pilT Legionella pneumophila strain Lp02

40.299

89.812

0.362
  pilT Legionella pneumophila strain ERS1305867

40.299

89.812

0.362
  pilT Acinetobacter baylyi ADP1

39.589

91.421

0.362