Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OCV04_RS13525 Genome accession   NZ_AP024893
Coordinates   2920373..2920972 (-) Length   199 a.a.
NCBI ID   WP_171138343.1    Uniprot ID   -
Organism   Vibrio plantisponsor strain CECT 7581     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2915373..2925972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV04_RS13505 aroB 2916279..2917364 (-) 1086 WP_171138345.1 3-dehydroquinate synthase -
  OCV04_RS13510 aroK 2917392..2917910 (-) 519 WP_042485096.1 shikimate kinase AroK -
  OCV04_RS13515 pilQ 2918097..2919842 (-) 1746 WP_102941191.1 type IV pilus secretin PilQ Machinery gene
  OCV04_RS13520 pilP 2919861..2920376 (-) 516 WP_171138344.1 pilus assembly protein PilP Machinery gene
  OCV04_RS13525 pilO 2920373..2920972 (-) 600 WP_171138343.1 type 4a pilus biogenesis protein PilO Machinery gene
  OCV04_RS13530 pilN 2920965..2921552 (-) 588 WP_171138342.1 PilN domain-containing protein Machinery gene
  OCV04_RS13535 pilM 2921542..2922552 (-) 1011 WP_102941195.1 type IV pilus assembly protein PilM Machinery gene
  OCV04_RS13540 - 2922696..2925173 (+) 2478 WP_171138341.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 23002.28 Da        Isoelectric Point: 4.7448

>NTDB_id=89228 OCV04_RS13525 WP_171138343.1 2920373..2920972(-) (pilO) [Vibrio plantisponsor strain CECT 7581]
MGDFKNLELDEILDWPRLPQLVMLLLAVVLIQGAGYWFYLVPKQDHLSALIEEEQTLKSTIQIKAHKAATLPQLKAQLDE
LSERYDYLLQQLPEQKELASMLAAVNELGIKHQLSFNRMDWGEKEQQAFLYRLPLNLELTGEYHHIGQFSQAIAQLPRII
YFENVDFQRISQDSQNLSVNVRAFTYQYQPDLPASGGQQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=89228 OCV04_RS13525 WP_171138343.1 2920373..2920972(-) (pilO) [Vibrio plantisponsor strain CECT 7581]
ATGGGTGATTTTAAAAACCTAGAGCTTGACGAAATATTGGATTGGCCGCGATTGCCACAGCTCGTGATGTTGCTGCTTGC
TGTCGTGCTGATTCAAGGTGCGGGATACTGGTTTTATCTTGTGCCCAAGCAAGACCACTTGAGCGCGCTCATTGAAGAAG
AACAAACGCTAAAATCCACCATTCAAATTAAAGCGCATAAAGCTGCAACTCTGCCGCAACTCAAAGCTCAACTCGATGAG
CTCTCAGAACGTTATGACTATTTGTTACAGCAACTGCCCGAGCAGAAAGAGCTGGCCAGTATGTTGGCTGCGGTGAATGA
GTTGGGCATCAAACATCAGCTGAGTTTCAATCGGATGGATTGGGGTGAAAAAGAACAACAAGCATTTTTATATCGCCTGC
CTCTGAATCTTGAGCTGACAGGTGAGTATCACCATATTGGCCAGTTCTCACAAGCCATCGCTCAGTTGCCAAGAATTATC
TATTTCGAGAACGTTGATTTTCAGCGAATCAGTCAGGACAGCCAGAATTTGAGTGTCAATGTACGAGCCTTTACCTATCA
GTATCAGCCTGATTTACCAGCCTCAGGAGGTCAGCAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

64.583

96.482

0.623
  pilO Vibrio campbellii strain DS40M4

63.684

95.477

0.608

Multiple sequence alignment