Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RGE70_RS13140 Genome accession   NZ_CP136522
Coordinates   3087071..3088108 (-) Length   345 a.a.
NCBI ID   WP_310471898.1    Uniprot ID   -
Organism   Shewanella sp. DAU334     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3082071..3093108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE70_RS13120 (RGE70_13115) xni 3083346..3084110 (+) 765 WP_310471894.1 flap endonuclease Xni -
  RGE70_RS13125 (RGE70_13120) - 3084214..3084603 (+) 390 WP_310471895.1 DUF3192 domain-containing protein -
  RGE70_RS13130 (RGE70_13125) hemH 3084694..3085707 (-) 1014 WP_310471896.1 ferrochelatase -
  RGE70_RS13135 (RGE70_13130) pilU 3085945..3087057 (-) 1113 WP_310471897.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RGE70_RS13140 (RGE70_13135) pilT 3087071..3088108 (-) 1038 WP_310471898.1 type IV pilus twitching motility protein PilT Machinery gene
  RGE70_RS13145 (RGE70_13140) - 3088144..3088833 (+) 690 WP_310471899.1 YggS family pyridoxal phosphate-dependent enzyme -
  RGE70_RS13150 (RGE70_13145) proC 3088989..3089807 (+) 819 WP_310471900.1 pyrroline-5-carboxylate reductase -
  RGE70_RS13155 (RGE70_13150) - 3089842..3090390 (+) 549 WP_310471901.1 YggT family protein -
  RGE70_RS13160 (RGE70_13155) yggU 3090390..3090677 (+) 288 WP_310472982.1 DUF167 family protein YggU -
  RGE70_RS13165 (RGE70_13160) - 3090760..3091194 (+) 435 WP_310471902.1 DUF4426 domain-containing protein -
  RGE70_RS13170 (RGE70_13165) rdgB 3091485..3092084 (+) 600 WP_310471903.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38330.88 Da        Isoelectric Point: 6.9096

>NTDB_id=891339 RGE70_RS13140 WP_310471898.1 3087071..3088108(-) (pilT) [Shewanella sp. DAU334]
MEITELLAFSVKHKASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGSAAVFRTIPSEVLSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQISREDAMLKSSNKQTNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=891339 RGE70_RS13140 WP_310471898.1 3087071..3088108(-) (pilT) [Shewanella sp. DAU334]
ATGGAAATCACAGAATTACTTGCCTTTAGTGTAAAGCACAAAGCATCAGATCTACACCTTTCTGCGGGGGTTTCTCCAAT
GATCCGTGTTGATGGTGAAGTTAGAAAGATTAATCTTCCTGCTTTAGACCATCAAGGTGTTCACAGTCTTGTTTACGATA
TAATGAATGATAAGCAGCGTAAGGATTACGAGGAGCATTTAGAGATCGATTTCTCGTTTGAAGTGCCTGGGCTTGCTCGT
TTTCGTGTCAATGCTTTTAATCAGTCCCGCGGCTCCGCGGCGGTGTTCCGTACTATTCCTAGCGAAGTCTTAAGCTTAGA
GCAACTGGGAGCTCCTGAGATTTTTAAGAAAATTTCGAGTTTTCCACGGGGGCTAGTGTTGGTTACCGGTCCAACTGGTT
CGGGTAAAAGTACCACCTTGGCTGCGATGGTTGATTACGTGAATGAAAATCGTCATGACCACATATTGACGATTGAAGAC
CCAATCGAATTTGTACATCAAAATAAACAGTGTTTGATTAACCAACGTGAAGTTCACCGTCATACTCACAGTTTTAATGC
TGCGCTGCGTAGTGCGTTACGTGAAGATCCCGATGTGATACTCGTAGGTGAAATGCGCGATTTGGAAACTATCAGATTAG
CGATGACAGCAGCTGAAACCGGTCACCTTGTATTTGGCACATTGCATACCACTTCTGCGGCTAAAACGATTGACCGTGTG
GTTGACGTGTTCCCCGAAGGTGAAAAGGGCATGGTGAGAACGATGCTGTCGGAATCTCTGCAAGCTGTTATCTCGCAAAC
TTTGATTAAAAAAGTGGGTGGAGGGCGTGTGGCCGCACATGAAATTATGATGGGCACTCCAGCTATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAAATGTACTCGGCGATTCAAACCGGTATGGCTCATGGTATGCAAACGCTGGATCAATGCCTA
CAGAATTTGGTTAACCGCGGTCAAATTAGCCGTGAAGATGCCATGCTGAAAAGCTCGAATAAGCAAACCAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.117

99.13

0.794
  pilT Acinetobacter baumannii D1279779

80.117

99.13

0.794
  pilT Acinetobacter baumannii strain A118

80.117

99.13

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Pseudomonas stutzeri DSM 10701

77.681

100

0.777
  pilT Acinetobacter baylyi ADP1

79.394

95.652

0.759
  pilT Legionella pneumophila strain Lp02

74.487

98.841

0.736
  pilT Legionella pneumophila strain ERS1305867

74.487

98.841

0.736
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.107

97.681

0.704
  pilT Vibrio cholerae strain A1552

72.107

97.681

0.704
  pilT Neisseria meningitidis 8013

68.895

99.71

0.687
  pilT Neisseria gonorrhoeae MS11

68.605

99.71

0.684
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.399

99.42

0.412
  pilU Vibrio cholerae strain A1552

41.369

97.391

0.403
  pilU Acinetobacter baylyi ADP1

39.481

100

0.397