Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RGE70_RS13135 Genome accession   NZ_CP136522
Coordinates   3085945..3087057 (-) Length   370 a.a.
NCBI ID   WP_310471897.1    Uniprot ID   -
Organism   Shewanella sp. DAU334     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3080945..3092057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE70_RS13115 (RGE70_13110) ppnN 3081997..3083349 (+) 1353 WP_310471893.1 nucleotide 5'-monophosphate nucleosidase PpnN -
  RGE70_RS13120 (RGE70_13115) xni 3083346..3084110 (+) 765 WP_310471894.1 flap endonuclease Xni -
  RGE70_RS13125 (RGE70_13120) - 3084214..3084603 (+) 390 WP_310471895.1 DUF3192 domain-containing protein -
  RGE70_RS13130 (RGE70_13125) hemH 3084694..3085707 (-) 1014 WP_310471896.1 ferrochelatase -
  RGE70_RS13135 (RGE70_13130) pilU 3085945..3087057 (-) 1113 WP_310471897.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RGE70_RS13140 (RGE70_13135) pilT 3087071..3088108 (-) 1038 WP_310471898.1 type IV pilus twitching motility protein PilT Machinery gene
  RGE70_RS13145 (RGE70_13140) - 3088144..3088833 (+) 690 WP_310471899.1 YggS family pyridoxal phosphate-dependent enzyme -
  RGE70_RS13150 (RGE70_13145) proC 3088989..3089807 (+) 819 WP_310471900.1 pyrroline-5-carboxylate reductase -
  RGE70_RS13155 (RGE70_13150) - 3089842..3090390 (+) 549 WP_310471901.1 YggT family protein -
  RGE70_RS13160 (RGE70_13155) yggU 3090390..3090677 (+) 288 WP_310472982.1 DUF167 family protein YggU -
  RGE70_RS13165 (RGE70_13160) - 3090760..3091194 (+) 435 WP_310471902.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41107.86 Da        Isoelectric Point: 5.5870

>NTDB_id=891338 RGE70_RS13135 WP_310471897.1 3085945..3087057(-) (pilU) [Shewanella sp. DAU334]
MDASPFLKTMIERKASDLFITVGFPPSAKIDGELVPLTEDIFTAEQSLQLVESLMTDVQKREFHESRECNFAFADKVYGR
FRVSAFWQRESPGCVMRRIETTIPEVDDLKLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPENKHAQLLFDLSLNLRGIVAQQLIPKSDGSGRRAAIEVLINTPRVASLIAKNELHSLKETMSKSNEQGMQTFDQA
LLRLYSDGEISYADALHHADSPNDLRLMIKLQSSDSSRSSSMDGVTLDFD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=891338 RGE70_RS13135 WP_310471897.1 3085945..3087057(-) (pilU) [Shewanella sp. DAU334]
ATGGATGCTAGTCCTTTTTTAAAGACCATGATCGAGCGTAAAGCGTCTGATTTGTTTATTACGGTAGGCTTTCCGCCTAG
CGCAAAGATTGATGGCGAATTAGTACCATTAACCGAGGACATTTTTACTGCAGAGCAGTCTCTTCAGTTAGTTGAATCAT
TAATGACGGATGTGCAAAAGCGTGAGTTTCATGAGTCTCGTGAATGTAACTTTGCTTTTGCCGATAAAGTATATGGACGT
TTTCGTGTCAGCGCTTTTTGGCAAAGAGAGTCGCCAGGTTGCGTAATGCGTCGTATTGAAACAACCATACCTGAAGTTGA
TGACTTAAAATTGCCGCCCATTCTTAAAGATCTCGTTATGAGTAAGCGAGGCTTAGTCATCATGGTTGGCGGAACCGGGA
CCGGTAAATCAACCTCATTAGCGGCGTTGGTCGGTTACCGTAATGCCCATGCTCGAGGGCACATTTTGACCATTGAAGAC
CCGGTGGAATTTGTGCATGACCATCGACAAAGTATCATCACTCAGCGAGAAGTTGGTATTGATACTGAGTCTTTTGATGC
CGCACTTAAAAGTTCGTTACGCCAAGCTCCGGATGTCATTTTAATTGGCGAGATCCGTACGCAAGAGACCATGGAGTTTG
CTTTGTCATTTGCTGAAACCGGTCACCTGTGTATGGCAACGTTGCATGCTAACAATGCTAACCAAGCACTTGATCGCATT
ATGCATTTAGTGCCTGAAAATAAGCATGCTCAGTTATTGTTTGATTTGTCGCTCAATTTACGCGGTATTGTGGCACAGCA
ATTGATTCCCAAAAGTGATGGTTCTGGCAGACGCGCGGCGATTGAGGTATTAATTAATACGCCGCGAGTGGCGAGCTTAA
TTGCTAAAAATGAGTTGCATTCATTGAAAGAAACCATGTCAAAGTCGAACGAGCAGGGCATGCAGACTTTTGATCAGGCG
TTACTAAGGTTATACAGTGACGGCGAAATTAGCTATGCCGATGCATTACATCATGCTGATTCACCAAATGATTTACGCTT
AATGATTAAACTGCAAAGTTCAGATTCAAGTCGCTCTAGTTCTATGGATGGGGTTACGCTTGATTTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.569

100

0.597
  pilU Vibrio cholerae strain A1552

58.108

100

0.581
  pilU Acinetobacter baylyi ADP1

60.227

95.135

0.573
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.739

93.243

0.389
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.189

0.373
  pilT Vibrio cholerae strain A1552

41.818

89.189

0.373
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362