Detailed information NTG0891222

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RTZ60_RS02465
• Genome accession: NZ_CP136504
• Coordinates: 539941..541005 (+)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae strain JZY2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 534941..546005

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RTZ60_RS02455 (RTZ60_000490)	-	535696..536964 (+)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-
RTZ60_RS02460 (RTZ60_000492)	-	537290..539734 (-)	2445	WP_017682643.1	penicillin-binding protein 1A	-
RTZ60_RS02465 (RTZ60_000493)	pilM	539941..541005 (+)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
RTZ60_RS02470 (RTZ60_000494)	-	541005..541631 (+)	627	WP_003378851.1	PilN domain-containing protein	-
RTZ60_RS02475 (RTZ60_000495)	pilO	541628..542251 (+)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
RTZ60_RS02480 (RTZ60_000496)	pilP	542248..542775 (+)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
RTZ60_RS02485 (RTZ60_000497)	pilQ	542823..544928 (+)	2106	WP_017682642.1	type IV pilus secretin PilQ	Machinery gene
RTZ60_RS02490 (RTZ60_000498)	aroK	544933..545451 (+)	519	WP_003378847.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=891222 RTZ60_RS02465 WP_003378852.1 539941..541005(+) (pilM) [Pseudomonas syringae pv. actinidiae strain JZY2]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=891222 RTZ60_RS02465 WP_003378852.1 539941..541005(+) (pilM) [Pseudomonas syringae pv. actinidiae strain JZY2]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTAGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469