Detailed information NTG0891190

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RTE98_RS03380
• Genome accession: NZ_CP136501
• Coordinates: 746229..747374 (-)
• Length: 381 a.a.
• NCBI ID: WP_026006585.1
• Uniprot ID: A0AA47I1L4
• Organism: Stutzerimonas frequens strain L27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 741229..752374

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RTE98_RS03355 (RTE98_03355)	-	741309..742325 (+)	1017	WP_017245430.1	aspartate carbamoyltransferase catalytic subunit	-
RTE98_RS03360 (RTE98_03360)	-	742325..743599 (+)	1275	WP_110778830.1	dihydroorotase	-
RTE98_RS03365 (RTE98_03365)	-	743656..744051 (-)	396	WP_017245432.1	TM2 domain-containing protein	-
RTE98_RS03370 (RTE98_03370)	-	744184..745398 (+)	1215	WP_316958606.1	putative DNA modification/repair radical SAM protein	-
RTE98_RS03375 (RTE98_03375)	-	745398..746222 (+)	825	WP_316958607.1	TIGR03915 family putative DNA repair protein	-
RTE98_RS03380 (RTE98_03380)	pilU	746229..747374 (-)	1146	WP_026006585.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RTE98_RS03385 (RTE98_03385)	pilT	747435..748469 (-)	1035	WP_017245436.1	type IV pilus twitching motility protein PilT	Machinery gene
RTE98_RS03390 (RTE98_03390)	-	748536..749225 (+)	690	WP_230724212.1	YggS family pyridoxal phosphate-dependent enzyme	-
RTE98_RS03395 (RTE98_03395)	proC	749236..750060 (+)	825	WP_200597334.1	pyrroline-5-carboxylate reductase	-
RTE98_RS03400 (RTE98_03400)	-	750070..750660 (+)	591	WP_017245439.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42540.84 Da
• Isoelectric Point: 6.8856

>NTDB_id=891190 RTE98_RS03380 WP_026006585.1 746229..747374(-) (pilU) [Stutzerimonas frequens strain L27]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTAMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTTVSQGLSLEVSDDDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=891190 RTE98_RS03380 WP_026006585.1 746229..747374(-) (pilU) [Stutzerimonas frequens strain L27]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGATCTGTTCATCACGGCGGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACCGCCATGTCACCGGAGATGACCCGCGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGTGCCCGCGGGATCGGTCGC
TTCCGTGTCAGTGCGTTCTATCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAGCTGAAGCTGCCTGACGTCCTGAAGAAGCTGTCGATGACCAAGCGTGGCCTGGTGCTGTTCGTCGGCGCCACCGGCA
CTGGCAAGTCGACCTCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCTAGCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATTCATCCACCAGCATCAGAACTGCATCGTTACCCAGCGCGAAGTCGGCATCGATACCAGCTCTTTCGAGGT
GGCACTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATTCTCATCGGCGAGATTCGTACCCGCGAGACGATGGACTATG
CCGTAGCCTTCGCCGAAACCGGACACCTGTGTCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCACCGGATCGGCACAACCAGGTCTGGATGGATCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAACA
GCTGGTGCCGACACCCGACGGCAAGGGTCGTCGCGCGGTGATCGAGGTATTGATCAACACCCCGCTGGCCGCGGACCTTA
TCCGCAAGGGCGAAGTGCATGAACTCAAGTCGCTGATGAAACGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTATGTGCAGGGCGAGATCACCTACGAAGATGCCCTGTTGCACGCCGACTCGGCCAACGACCTGCGGCT
GTTGATCAAGCTGGGTTCGGAGACCGACGGTGAGCACCTGACCACTGTTTCGCAGGGGTTGAGTCTGGAGGTCAGCGACG
ACGATCCGGGTCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	94.751	100	0.948
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.714	91.864	0.549
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.658	88.976	0.388