Detailed information NTG0890582

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAFA16_RS03875
• Genome accession: NZ_CP151715
• Coordinates: 811486..812604 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain F15-01B
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 806486..817604

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAFA16_RS03845 (AAFA16_03845)	-	807316..808164 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
AAFA16_RS03850 (AAFA16_03850)	-	808416..808871 (+)	456	WP_000782976.1	bacteriohemerythrin	-
AAFA16_RS03855 (AAFA16_03855)	-	809024..810091 (+)	1068	WP_004736014.1	hypothetical protein	-
AAFA16_RS03860 (AAFA16_03860)	-	810091..810414 (+)	324	WP_000442582.1	RnfH family protein	-
AAFA16_RS03865 (AAFA16_03865)	bamE	810469..810867 (-)	399	WP_001170993.1	outer membrane protein assembly factor BamE	-
AAFA16_RS03870 (AAFA16_03870)	fur	810979..811416 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AAFA16_RS03875 (AAFA16_03875)	pilU	811486..812604 (-)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAFA16_RS03880 (AAFA16_03880)	pilT	812632..813669 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
AAFA16_RS03885 (AAFA16_03885)	-	813796..814488 (+)	693	WP_001108513.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=890582 AAFA16_RS03875 WP_000347039.1 811486..812604(-) (pilU) [Acinetobacter baumannii strain F15-01B]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=890582 AAFA16_RS03875 WP_000347039.1 811486..812604(-) (pilU) [Acinetobacter baumannii strain F15-01B]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395