Detailed information NTG0889734

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RVY87_RS00445
• Genome accession: NZ_CP136240
• Coordinates: 92422..93543 (-)
• Length: 373 a.a.
• NCBI ID: WP_022997479.1
• Uniprot ID: A0A9Q3ZEX2
• Organism: Alloalcanivorax xenomutans strain HF-10
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 87422..98543

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RVY87_RS00420 (RVY87_00420)	-	87561..88454 (-)	894	WP_026948838.1	LysR family transcriptional regulator	-
RVY87_RS00425 (RVY87_00425)	-	88552..89160 (+)	609	WP_063141396.1	NAD(P)H-dependent oxidoreductase	-
RVY87_RS00430 (RVY87_00430)	-	89208..90212 (+)	1005	WP_026948839.1	glutathione S-transferase family protein	-
RVY87_RS00435 (RVY87_00435)	-	90553..91407 (+)	855	WP_116027484.1	pirin family protein	-
RVY87_RS00440 (RVY87_00440)	-	91492..91806 (-)	315	WP_262459886.1	HigA family addiction module antitoxin	-
RVY87_RS00445 (RVY87_00445)	pilU	92422..93543 (-)	1122	WP_022997479.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RVY87_RS00450 (RVY87_00450)	pilT	93562..94596 (-)	1035	WP_022997480.1	type IV pilus twitching motility protein PilT	Machinery gene
RVY87_RS00455 (RVY87_00455)	-	94715..95419 (+)	705	WP_063141403.1	YggS family pyridoxal phosphate-dependent enzyme	-
RVY87_RS00460 (RVY87_00460)	proC	95452..96264 (+)	813	WP_022997482.1	pyrroline-5-carboxylate reductase	-
RVY87_RS00465 (RVY87_00465)	-	96878..97426 (+)	549	WP_022997221.1	YggT family protein	-
RVY87_RS00470 (RVY87_00470)	-	97504..98427 (-)	924	WP_022997222.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41549.72 Da
• Isoelectric Point: 6.7399

>NTDB_id=889734 RVY87_RS00445 WP_022997479.1 92422..93543(-) (pilU) [Alloalcanivorax xenomutans strain HF-10]
MEFEDLLKLMVQKGGSDLFITAGVPPSMKVHGKVVPVTRNALTPDMTRDLVFGVMSEKQRKEFVDQHECNFALSSRGIGR
FRVSAFYQRNLVGMVLRRIETKIPDADELRLPPIIKDLAMTKRGLVIFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGLDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLRGIVAQQLIPTPDGKGRRAAIEVLLNTPLVADHIRKGEVHLLKALMGKSREQGMQTFDQA
LYDLYDAGEITYEDAIAHADSPNDLRLMIKLANETDASSLDHATRGLSIEDSD

Nucleotide

• Length: 1122 bp

>NTDB_id=889734 RVY87_RS00445 WP_022997479.1 92422..93543(-) (pilU) [Alloalcanivorax xenomutans strain HF-10]
ATGGAATTCGAAGATCTGCTCAAACTGATGGTGCAAAAAGGCGGCTCGGACTTGTTCATCACCGCCGGTGTTCCACCAAG
TATGAAAGTCCACGGCAAGGTGGTGCCGGTTACCCGTAACGCCCTGACCCCGGACATGACCCGGGATCTGGTGTTCGGTG
TGATGTCGGAAAAGCAGCGCAAGGAGTTCGTCGACCAGCACGAATGCAATTTCGCGCTATCCTCGCGGGGCATTGGCCGC
TTCCGTGTGTCCGCTTTTTACCAGCGCAATCTGGTGGGCATGGTGTTGCGGCGCATCGAGACCAAGATACCCGACGCCGA
CGAACTGCGGCTGCCGCCGATCATCAAGGACCTGGCGATGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGAAAATCCACCTCCCTGGCGTCAATGATCGGCCACCGTAATCGCAACTCCAAAGGCCATATCATCTCCATTGAGGAT
CCGATCGAATTCATCCACCAGCATCAGGGCTGCATTGTCACGCAACGGGAAGTAGGCCTGGATACGGACAGTTTCGAGAT
CGCCCTCAAGAACACGCTGCGACAGGCACCGGATGTGATTCTGATCGGTGAGATCCGCAGCCGCGAAGTGATGGATTACG
CCATTGCCTTCGCCGAAACCGGCCATCTGTGTCTGGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATTCACTTTTTCCCCGCCGACCGTCACCGGCAGTTATGGATGGACCTCTCCCTGAACCTGCGCGGCATCGTCGCCCAGCA
ACTGATCCCGACGCCGGACGGCAAGGGCCGCCGCGCCGCCATCGAAGTGCTGCTGAACACGCCACTGGTGGCGGACCATA
TCCGTAAAGGCGAAGTGCATTTGCTCAAGGCGCTGATGGGCAAATCCCGGGAGCAGGGCATGCAGACTTTCGATCAGGCC
CTCTATGATCTTTATGATGCCGGCGAAATCACTTACGAGGACGCGATTGCCCACGCCGACTCCCCCAACGATCTGCGCCT
GATGATCAAGCTCGCCAACGAAACCGACGCCTCCAGCCTGGACCACGCCACCCGTGGCCTGAGCATCGAGGACTCGGACT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	76.944	100	0.769
pilU	Acinetobacter baylyi ADP1	67.385	99.464	0.67
pilU	Vibrio cholerae strain A1552	56.267	96.247	0.542
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.017	95.71	0.402