Detailed information NTG0889521

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWU47_RS13730
• Genome accession: NZ_CP136180
• Coordinates: 2897965..2899083 (+)
• Length: 372 a.a.
• NCBI ID: WP_317856483.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain ABO21-A020
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2892965..2904083

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWU47_RS13720 (RWU47_13720)	-	2896081..2896773 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
RWU47_RS13725 (RWU47_13725)	pilT	2896900..2897937 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWU47_RS13730 (RWU47_13730)	pilU	2897965..2899083 (+)	1119	WP_317856483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWU47_RS13735 (RWU47_13735)	fur	2899153..2899590 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWU47_RS13740 (RWU47_13740)	-	2899702..2900100 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWU47_RS13745 (RWU47_13745)	-	2900155..2900478 (-)	324	WP_000442587.1	RnfH family protein	-
RWU47_RS13750 (RWU47_13750)	-	2900478..2901545 (-)	1068	WP_002019572.1	hypothetical protein	-
RWU47_RS13755 (RWU47_13755)	-	2901698..2902153 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RWU47_RS13760 (RWU47_13760)	-	2902406..2903254 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41947.15 Da
• Isoelectric Point: 6.6945

>NTDB_id=889521 RWU47_RS13730 WP_317856483.1 2897965..2899083(+) (pilU) [Acinetobacter baumannii strain ABO21-A020]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFEQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889521 RWU47_RS13730 WP_317856483.1 2897965..2899083(+) (pilU) [Acinetobacter baumannii strain ABO21-A020]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGAGCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392