Detailed information NTG0889384

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWV42_RS14335
• Genome accession: NZ_CP136173
• Coordinates: 3027218..3028336 (+)
• Length: 372 a.a.
• NCBI ID: WP_318590129.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain ABO21-A049
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3022218..3033336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWV42_RS14325 (RWV42_14325)	-	3025334..3026026 (-)	693	WP_001108515.1	YggS family pyridoxal phosphate-dependent enzyme	-
RWV42_RS14330 (RWV42_14330)	pilT	3026153..3027190 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWV42_RS14335 (RWV42_14335)	pilU	3027218..3028336 (+)	1119	WP_318590129.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWV42_RS14340 (RWV42_14340)	fur	3028406..3028843 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWV42_RS14345 (RWV42_14345)	-	3028955..3029353 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWV42_RS14350 (RWV42_14350)	-	3029408..3029731 (-)	324	WP_000442585.1	RnfH family protein	-
RWV42_RS14355 (RWV42_14355)	-	3029731..3030798 (-)	1068	WP_029776981.1	membrane protein	-
RWV42_RS14360 (RWV42_14360)	-	3030951..3031406 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RWV42_RS14365 (RWV42_14365)	-	3031659..3032507 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.17 Da
• Isoelectric Point: 6.6944

>NTDB_id=889384 RWV42_RS14335 WP_318590129.1 3027218..3028336(+) (pilU) [Acinetobacter baumannii strain ABO21-A049]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNVNQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889384 RWV42_RS14335 WP_318590129.1 3027218..3028336(+) (pilU) [Acinetobacter baumannii strain ABO21-A049]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TTATGAGCGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAATTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAATGTCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCACAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGTTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39