Detailed information NTG0889244

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWT88_RS14270
• Genome accession: NZ_CP136169
• Coordinates: 2938147..2939265 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain ABO21-A058
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2933147..2944265

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWT88_RS14260 (RWT88_14260)	-	2936263..2936955 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
RWT88_RS14265 (RWT88_14265)	pilT	2937082..2938119 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWT88_RS14270 (RWT88_14270)	pilU	2938147..2939265 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWT88_RS14275 (RWT88_14275)	fur	2939335..2939772 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWT88_RS14280 (RWT88_14280)	-	2939884..2940282 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWT88_RS14285 (RWT88_14285)	-	2940337..2940660 (-)	324	WP_000442587.1	RnfH family protein	-
RWT88_RS14290 (RWT88_14290)	-	2940660..2941727 (-)	1068	WP_002019572.1	hypothetical protein	-
RWT88_RS14295 (RWT88_14295)	-	2941880..2942335 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RWT88_RS14300 (RWT88_14300)	-	2942588..2943436 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=889244 RWT88_RS14270 WP_000347039.1 2938147..2939265(+) (pilU) [Acinetobacter baumannii strain ABO21-A058]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889244 RWT88_RS14270 WP_000347039.1 2938147..2939265(+) (pilU) [Acinetobacter baumannii strain ABO21-A058]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395