Detailed information NTG0889103

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RWX82_RS13745
• Genome accession: NZ_CP136166
• Coordinates: 2899352..2900470 (+)
• Length: 372 a.a.
• NCBI ID: WP_317856483.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain ABO21-A063
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2894352..2905470

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RWX82_RS13735 (RWX82_13735)	-	2897468..2898160 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
RWX82_RS13740 (RWX82_13740)	pilT	2898287..2899324 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RWX82_RS13745 (RWX82_13745)	pilU	2899352..2900470 (+)	1119	WP_317856483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RWX82_RS13750 (RWX82_13750)	fur	2900540..2900977 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RWX82_RS13755 (RWX82_13755)	-	2901089..2901487 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RWX82_RS13760 (RWX82_13760)	-	2901542..2901865 (-)	324	WP_000442587.1	RnfH family protein	-
RWX82_RS13765 (RWX82_13765)	-	2901865..2902932 (-)	1068	WP_002019572.1	hypothetical protein	-
RWX82_RS13770 (RWX82_13770)	-	2903085..2903540 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RWX82_RS13775 (RWX82_13775)	-	2903793..2904641 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41947.15 Da
• Isoelectric Point: 6.6945

>NTDB_id=889103 RWX82_RS13745 WP_317856483.1 2899352..2900470(+) (pilU) [Acinetobacter baumannii strain ABO21-A063]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFEQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=889103 RWX82_RS13745 WP_317856483.1 2899352..2900470(+) (pilU) [Acinetobacter baumannii strain ABO21-A063]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGAGCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392