Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RUI02_RS12365 Genome accession   NZ_CP136099
Coordinates   2404062..2405132 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus sp. TSA-4     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2399062..2410132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RUI02_RS12320 (RUI02_12320) tapA 2399540..2400301 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  RUI02_RS12325 (RUI02_12325) - 2400573..2400899 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  RUI02_RS12330 (RUI02_12330) - 2400941..2401120 (-) 180 WP_029726723.1 YqzE family protein -
  RUI02_RS12335 (RUI02_12335) comGG 2401192..2401566 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  RUI02_RS12340 (RUI02_12340) comGF 2401567..2401950 (-) 384 WP_046160582.1 ComG operon protein ComGF Machinery gene
  RUI02_RS12345 (RUI02_12345) comGE 2401976..2402323 (-) 348 WP_046381178.1 ComG operon protein 5 Machinery gene
  RUI02_RS12350 (RUI02_12350) comGD 2402307..2402738 (-) 432 WP_046381179.1 comG operon protein ComGD Machinery gene
  RUI02_RS12355 (RUI02_12355) comGC 2402728..2403024 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  RUI02_RS12360 (RUI02_12360) comGB 2403038..2404075 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  RUI02_RS12365 (RUI02_12365) comGA 2404062..2405132 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  RUI02_RS12370 (RUI02_12370) - 2405344..2405541 (-) 198 WP_014480259.1 CBS domain-containing protein -
  RUI02_RS12375 (RUI02_12375) corA 2405543..2406496 (-) 954 WP_004399136.1 magnesium transporter CorA -
  RUI02_RS12380 (RUI02_12380) - 2406639..2407967 (+) 1329 WP_003230150.1 hemolysin family protein -
  RUI02_RS12385 (RUI02_12385) rsbRD 2408020..2408856 (-) 837 WP_046381180.1 RsbT co-antagonist protein RsbRD -
  RUI02_RS12395 (RUI02_12395) mgsR 2409080..2409460 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  RUI02_RS12400 (RUI02_12400) - 2409692..2409937 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=888420 RUI02_RS12365 WP_004399124.1 2404062..2405132(-) (comGA) [Bacillus sp. TSA-4]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=888420 RUI02_RS12365 WP_004399124.1 2404062..2405132(-) (comGA) [Bacillus sp. TSA-4]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAAGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1